Yeast “microbreweries” within disposable badges could help hospital lab workers track daily exposure to radiation, which would allow faster assessment of tissue damage that could lead to cancer.
Made from freezer paper, aluminum, and tape, the patch works by simply adding a drop of water to activate the yeast to show radiation exposure, which an electronic device can read.
On a commercial level, the readout device could one day be a tablet or phone. Researchers could also adapt the badge in the future for nuclear power plant workers and victims of nuclear disasters.
“You would use the badge when you’re in the lab and recycle it after you’ve checked your exposure by plugging it into a device,” says Manuel Ochoa, a postdoctoral researcher in the School of Electrical and Computer Engineering at Purdue University.
Radiology workers are regularly exposed to low doses of radiation when they obtain patient imagery, such as X-rays. While protective gear largely keeps workers within a safe range of radiation exposure, absorbing a little bit is still inevitable.
Radiation doses creeping above regulated guidelines pose risk for developing conditions such as cancer, cataracts, skin irritation, or thyroid disease.
“Currently, radiology workers are required to wear badges, called dosimeters, on various parts of their bodies for monitoring their radiation exposure,” says Babak Ziaie, professor of electrical and computer engineering. “They wear the badges for a month or two, and then they send them to the company that made them. But it takes weeks for the company to read the data and send a report back to the hospital. Ours give an instant reading at much lower cost.”
The success of the badge lies in the quick and measurable response of yeast to radiation: The higher the radiation dose, the higher the percentage of yeast cells that die. Wetting the badge activates the cells that are still alive to eat glucose and release carbon dioxide—the same fermentation process responsible for brewing beer and making bread rise.
When carbon dioxide bubbles at the surface, ions also form. The concentration of these ions increases the electrical conductivity of yeast, which can be measured by hooking up the badge to a readout system.
“We use the change in electrical properties of the yeast to tell us how much radiation damage it incurred. A slow decrease in electrical conductivity over time indicates more damage,” says Rahim Rahimi, postdoctoral researcher in electrical and computer engineering.
Numbers from the readout system translate to rads—the units used by entities like the Occupational Safety and Health Administration to specify limits on how much radiation human tissue can safely absorb. Skin of the whole body, for example, shouldn’t be exposed to more than 7.5 rad over a three-month period.
The new device can detect a radiation dose as little as 1 millirad in the yeast badges, which is comparable to current commercial badges.
Yeast is also known to be genetically similar to human tissue. Data from the badges can, therefore, inform future work on how radiation damage happens to human DNA and proteins.
“For yeast, it seems that radiation primarily affects the cell walls of the membrane and mitochondria,” Ochoa says. “Since biologists are already familiar with yeast, then we’re more likely to understand what’s causing the biological effects of radiation in organic matter.”
The findings appear in Advanced Biosystems. A patent is pending for the technology via the Purdue Research Foundation. NextFlex funded the work.
Source: Purdue University