A simple skin test can accurately identify Parkinson’s disease, researchers report.
Physicians currently diagnose patients using clinical signs and symptoms but a definitive diagnosis can only come during an autopsy. Parkinson’s disease is commonly misdiagnosed early, complicating clinical trials of potential treatments.
The study, published in the journal Movement Disorders, shows how a chemical assay can detect clumping of the protein alpha-synuclein in skin samples to help diagnose Parkinson’s disease. Using the assay can lead to earlier detection of Parkinson’s and better clinical trials, the researchers say.
“Since there’s no easy and reliable test available for the early diagnosis of Parkinson’s disease at present, we think there will be a lot interest in the potential use of skin samples for diagnosis,” says lead author Anumantha Kanthasamy, chair and professor of biomedical sciences at Iowa State University.
The researchers conducted a blinded study of 50 skin samples from the Arizona Study of Aging and Neurodegenerative Disorders (AZSAND)/Brain and Body Donation Program based at Banner Sun Health Research Institute. Half of the skin samples came from patients with Parkinson’s disease and half came from people without neurologic disease.
Using the protein assay correctly diagnosed 24 out of 25 Parkinson’s disease patients and only 1 out of 25 controls had the protein clumping.
“These results indicate tremendously high sensitivity and specificity which is critical for a diagnostic test,” says Charles Adler, professor of neurology at the Mayo Clinic Arizona, and a co-investigator of the study.
“The clinical diagnostic accuracy for early-stage PD has been quite poor, only around 50-70%. And since clinical trials really need to be done at an early stage to avoid further brain damage, they have been critically hampered because they have been including large percentages of people who may not actually have the disease,” says Thomas Beach, a co-investigator of the study and head of the Civin Laboratory at Banner Sun Health Research Institute.
“Improving clinical diagnostic accuracy is, in my view, the very first thing we need to do in order to find new useful treatments for PD,” Beach says.
The research centers on a method known as the real-time quaking induced conversion assay, a test originally developed to detect mad cow disease. Kanthasamy’s laboratory has spent several years optimizing the assay for detecting misfolded proteins in similar human and animal disorders.
Parkinson’s disease arises from misfolded alpha-synuclein proteins that accumulate in the brain leading to neuronal damage. Adler and Beach have led research in AZSAND that found these misfolded alpha-synuclein proteins also collect in other body tissues as well, including the skin.
Testing skin samples could lead to earlier detection of Parkinson’s disease, Kanthasamy says, adding that earlier diagnosis could allow physicians to test therapeutic strategies designed to slow or prevent the development of advanced symptoms.
Source: Iowa State University