Hydrogel LEGO blocks stick together for microfluidics

(Credit: Getty Images)

Scientists have used 3D printing to pattern modular hydrogel components that can bend, twist, or stick together in response to certain chemicals.

For a paper in Polymer Chemistry, researchers demonstrated a soft gripper capable of actuating on demand to pick up small objects. They also designed LEGO-like hydrogel building blocks that can assemble then tightly seal together to form customized microfluidic devices—”lab-on-a-chip” systems used for drug screening, cell cultures, and other applications.

time-lapse of soft gripper
Time-lapse video of the actuation of a soft gripper. (Credit: Brown)

The key to the new material’s functionality is its dual polymer composition, researchers say.

“Essentially, the one polymer provides structural integrity, while the other enables these dynamic behaviors like bending or self-adhesion,” says lead author Thomas Valentin, a recently graduated PhD student in Brown University’s School of Engineering. “So putting the two together makes a material that’s greater than the sum of its parts.”

Gripper ‘fingers’

Hydrogels solidify when the polymer strands within them become tethered to each other, a process called crosslinking. Two types of bonds hold crosslinked polymers together: covalent and ionic.

Covalent bonds are quite strong, but irreversible. Once two strands link covalently, it’s easier to break the strand than it is to break the bond. Ionic bonds on the other hand are not quite as strong, but are reversible. Adding ions (atoms or molecules with a net positive or negative charge) will cause the bonds to form. Removing ions will cause the bonds to fall apart.

For the new material, researchers combined one polymer that’s covalently crosslinked, called PEGDA, and one that’s ionically crosslinked, called PAA.

The PEGDA’s strong covalent bonds hold the material together, while the PAA’s ionic bonds make it responsive. Putting the material in an ion-rich environment causes the PAA to crosslink, meaning it becomes more rigid and contracts. Take those ions away, and the material softens and swells as the ionic bonds break. The same process also makes the material self-adhesive when desired. Put two separate pieces together, add some ions, and the pieces attach tightly together.

That combination of strength and dynamic behavior enabled researchers to make their soft gripper. They patterned each of the gripper’s “fingers” to have pure PEGDA on one side and a PEGDA-PAA mixture on the other.

Adding ions caused the PEGDA-PAA side to shrink and strengthen, which pulled the two gripper fingers together. The researchers showed that the setup was strong enough to lift small objects weighing about a gram, and hold them against gravity.

LEGO blocks for microfluidics

“There’s a lot of interest in materials that can change their shapes and automatically adapt to different environments,” says corresponding author Ian Y. Wong, an assistant professor of engineering. “So here we demonstrate a material the can flex and reconfigure itself in response to an external stimulus.”

hydrogel LEGO become microfluidic devices
LEGO-like hydrogel building blocks patterned with tiny fluid channels can become complex microfluidic devices and then seal tightly together.

But potentially a more immediate application is in microfluidics, the researchers say.

Hydrogels are an attractive material for microfluidic devices, especially those used in biomedical testing. They’re soft and flexible like human tissue, and generally nontoxic. The problem is that hydrogels are often difficult to pattern with the complex channels and chambers needed in microfluidics.

But this new material—and the LEGO block concept it enables—offers a potential solution. The 3D printing process allows complex microfluidic architectures to incorporate into each block. Those blocks can then assemble using a socket configuration much like that of real LEGO blocks. Adding ions to the assembled blocks makes a water-tight seal.

“The modular LEGO blocks are interesting in that we could create a prefabricated toolbox for microfluidic devices,” Valentin says. “You keep a variety of preset parts with different microfluidic architectures on hand, and then you just grab the ones you need to make your custom microfluidic circuit. Then you heal them together and it’s ready to go.”

Storing the blocks for long periods before use doesn’t appear to pose a problem, the researchers say.

“Some of the samples we tested for this study were three or four months old,” says coauthor Eric DuBois, an undergraduate student. “So we think these could remain usable for an extended period.”

The researchers say they’ll continue to work with the material, potentially tweaking the properties of the polymers to get even more durability and functionality.

The US Department of Education and Brown University funded the work.

Source: Brown University