Chip splits cancer cells from blood for better analysis

HydroSeq, a new microfluidic chip designed to catch circulating tumor cells (CTCs) and isolate their RNA in order to develop better cancer treatments. (Credit: Yoon Lab/Michigan Engineering)

A new way to cleanly separate out cancer cells from a blood sample enables comprehensive genetic profiling of the cancer cells, according to a new study.

Genetic profiling of cancer cells could help doctors target tumors and monitor treatments more effectively. The new method is a dramatic improvement over current approaches because it also encompasses the variation among cancer cells within a single patient.

“This could be a whole different ball game,” says Max Wicha, professor of oncology at the University of Michigan and senior physician of the study in Nature Communications.

Earlier techniques meant a trade-off between a comprehensive genetic profile of a limited subset of cancer cells, or capturing most of the cancer cells and only being able to look for a few genes. As a result, the genetic profiles often neglected important populations of cancer cells—including cells believed to spread cancer in the body.

hydro-seq chip scale
At just bigger than a penny, a new lab-on-a-chip called Hydro-Seq enables comprehensive genetic profiling of cancer from a blood sample. (Credit: Yoon Lab/Michigan Engineering)

“Our chip allows us to capture pure circulating tumor cells and then extract genetic information without any contamination from red and white blood cells,” says senior author Euisik Yoon, professor of electrical engineering and computer science at University of Michigan.

Avoiding invasive biopsies

Many modern cancer drugs work by going after cells with certain genes in play—genes that flag their identities as cancer cells. But these genes aren’t uniformly active in a patient’s cancer cell population and can change over the course of treatment.

Repeated biopsies to monitor the tumor are painful and potentially dangerous for the patient. Capturing cancer cells from blood samples offers a noninvasive way to observe whether the cancer is disappearing or whether it is becoming resistant to the treatment.

“It allows you not only to select targeted therapies, but to monitor the effects of these therapies in patients by doing this blood test,” Wicha says.

Using this method, the team collected and analyzed 666 cancer cells from the blood of 21 breast cancer patients.

A cancer cell, in red, which Hydro-Seq captured from a blood sample. (Credit: Yoon Lab/Michigan Engineering)

The genetic analysis confirmed that even within a single patient, the cancer cells often behave very differently. Wicha’s group has previously shown that cancer cells with the properties of stem cells mediate metastasis.

Although cancer stem cells make up only a few percent of a tumor’s cells, they make up a higher proportion of the cancer cells in the bloodstream. In this study, about 30-50 percent of the cancer cells captured from the blood samples displayed stem-like properties.

This population is particularly easy to miss with techniques that capture clean-but-incomplete samples of cancer cells from patient blood by grabbing onto proteins on the cells’ surfaces. Stem-like cells are on a spectrum between two more-typical cell types, which means that they don’t display consistent protein markers.

To get a clean and unbiased set of cancer cells from a vial of blood, the team started with a technique that removes blood cells by sorting the blood sample according to cell size. Starting with about one cancer cell in a billion blood cells, this step left only about 95 or so blood cells for every cancer cell. But that’s still far too contaminated for a detailed genetic analysis.

The new method, which the researchers call Hydro-Seq, gets rid of those last blood cells and then analyzes each cell.

Chambers on a chip

The key technology is a chip with a system of channels and chambers. It traps cancer cells one at a time by drawing fluid through a drain in each chamber, and then plugs it when a cancer cell arrives. Once the chamber is plugged, cells in the channel pass it by and get sucked into the next chamber. Then, to “wash” the blood cells off the chip, they ran clean fluid backward through the chip and drew it out again, taking nearly all the rest of the contaminating cells along.

With a clean sample of isolated cancer cells, the team did the genetic profiles. They went after the cells’ “transcriptomes”—basically, snapshots of what DNA each cell was reading and using. This revealed the cells’ active genes.

The researchers captured the transcriptomes with barcoded beads, a method that until now was difficult to use with small cell samples. The team dropped a barcoded bead into each chamber and then closed the chambers before destroying the cell membranes. This released the RNA—the little bits of genetic code recently read from the cell’s DNA—so that the RNA attached to barcoded genetic code on the bead. The team could then analyze the contents of each cell separately.

“Before, we could measure two or three genes at a time with staining methods, but now we get a comprehensive picture of circulating tumor cells by measuring thousands of genes in each cell at once,” says co-first author Yu-Chih Chen, assistant research scientist in electrical engineering and computer science.

Cancer treatment can be a moving target, with cancers changing their gene expression as drugs kill off some cells but not others. Coauthor Monika Burness, assistant professor of internal medicine, expects to use the new device to track the progress of patients in an upcoming drug trial.

“It’s a very powerful tool to monitor—at the cellular level—what a treatment does to tumors over time,” says Burness, who studies new drug therapies for cancer patients.

Funding for the work came from the National Institutes of Health, Breast Cancer Research Foundation, Forbes Institute for Cancer Discovery, and the University of Michigan’s Coulter Translational Research Partnership Program. The researchers made the devices at the Lurie Nanofabrication Facility.

Source: University of Michigan