RICE (US) — Activated by a pulse of laser light, nanobubbles can kill diseased cells while leaving healthy cells untouched.
This unique use for tunable plasmonic nanobubbles, developed in the Rice University lab of Dmitri Lapotko, shows promise to replace several difficult processes now used to treat cancer patients, among others, with a fast, simple, multifunctional procedure.
The research is the focus of a paper published in the journal ACS Nano.
Plasmonic nanobubbles that are 10,000 times smaller than a human hair cause tiny explosions. The bubbles form around plasmonic gold nanoparticles that heat up when excited by an outside energy source—in this case, a short laser pulse—and vaporize a thin layer of liquid near the particle’s surface. The vapor bubble quickly expands and collapses.
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Lapotko and colleagues had already found that plasmonic nanobubbles kill cancer cells by literally exploding them without damage to healthy neighbors, a process that showed much higher precision and selectivity compared with those mediated by gold nanoparticles alone, he adds.
The new project takes that remarkable ability a few steps further.
A series of experiments proved a single laser pulse creates large plasmonic nanobubbles around hollow gold nanoshells, and these large nanobubbles selectively destroy unwanted cells.
The same laser pulse creates smaller nanobubbles around solid gold nanospheres that punch a tiny, temporary pore in the wall of a cell and create an inbound nanojet that rapidly “injects” drugs or genes into the other cells.
In their experiments, Lapotko and his team placed 60-nanometer-wide hollow nanoshells in model cancer cells and stained them red. In a separate batch, they put 60-nanometer-wide nanospheres into the same type of cells and stained them blue.
After suspending the cells together in a green fluorescent dye, they fired a single wide laser pulse at the combined sample, washed the green stain out and checked the cells under a microscope.
The red cells with the hollow shells were blasted apart by large plasmonic nanobubbles. The blue cells were intact, but green-stained liquid from outside had been pulled into the cells where smaller plasmonic nanobubbles around the solid spheres temporarily pried open the walls.
Because all of this happens in a fraction of a second, as many as 10 billion cells per minute could be selectively processed in a flow-through system like that under development at Rice, says Lapotko, a faculty fellow in biochemistry and cell biology and in physics and astronomy. That has potential to advance cell and gene therapy and bone marrow transplantation, he adds.
Most disease-fighting and gene therapies require “ex vivo”—outside the body—processing of human cell grafts to eliminate unwanted (like cancerous) cells and to genetically modify other cells to increase their therapeutic efficiency, explains Lapotko, the study’s lead author.
“Current cell processing is often slow, expensive and labor intensive and suffers from high cell losses and poor selectivity. Ideally both elimination and transfection (the introduction of materials into cells) should be highly efficient, selective, fast, and safe.”
Plasmonic nanobubble technology promises “a method of doing multiple things to a cell population at the same time,” says Malcolm Brenner, a professor of medicine and of pediatrics at Baylor College of Medicine, who collaborates with the Rice team.
“For example, if I want to put something into a stem cell to make it turn into another type of cell, and at the same time kill surrounding cells that have the potential to do harm when they go back into a patient—or into another patient—these very tunable plasmonic nanobubbles have the potential to do that,” adds Brenner.
Lapotko plans to build a prototype of the technology with an eye toward testing with human cells in the near future.
“We’d like for this to be a universal platform for cell and gene therapy and for stem cell transplantation,” he says.
The National Institutes of Health supported the work, which is a collaboration between Rice, Baylor College of Medicine, Texas Children’s Hospital, and the University of Texas MD Anderson Cancer Center.
Source: Rice University
