High doses of vitamin D may be an effective way to for people to fight infections like HIV-1.
“Vitamin D may be a simple, cost-effective intervention, particularly in resource-poor settings, to reduce HIV-1 risk and disease progression,” researchers write in a new study published online in the Proceedings of the National Academy of Sciences.
For the study, researchers looked at two ethnic groups in Cape Town, South Africa, to see how seasonal differences in exposure to ultraviolet B radiation, dietary vitamin D, genetics, and pigmentation affected vitamin D levels, and whether high-dose supplementation improved deficiencies and the cell’s ability to repel HIV-1.
“Cape Town, South Africa, has a seasonal ultraviolet B regime and one of the world’s highest rates of HIV-1 infection, peaking in young adults, making it an appropriate location for a longitudinal study like this one,” says Nina Jablonski, professor of anthropology at Penn State.
Winter decline
One hundred healthy young individuals divided between those of Xhosa ancestry—whose ancestors migrated from closer to the equator into the Cape area—and those self-identified as having Cape Mixed ancestry—a complex admixture of Xhosa, Khoisan, European, South Asian, and Indonesian populations—were recruited for this study.
The groups were matched for age and smoking. The Xhosa, whose ancestors came from a place with more ultraviolet B radiation, have the darkest skin pigmentation, while the Khoisan—the original inhabitants of the Cape—have adapted to the seasonally changing ultraviolet radiation in the area and are lighter skinned. The Cape Mixed population falls between the Xhosa and Khoisan in skin pigmentation levels.
Cape Town is situated in the southern hemisphere at about the same distance from the equator as the Florida panhandle, slightly more than 30 degrees latitude. Ultraviolet B levels show a winter decline anywhere above 30 degrees latitude, so Cape Town has a definite winter with low levels of the ultraviolet B wavelengths needed to produce precursor vitamin D3.
Add to this the fact that people now spend more time indoors during winter and wear more clothing, and exposure to ultraviolet B in winter may be insufficient to prevent vitamin D deficiency.
Skin pigment
Sunscreen use is not a factor in these populations. However, the darker the skin’s pigment, the more ultraviolet B radiation necessary to trigger the precursor chemicals in the body to produce vitamin D.
“The skin of the indigenous people of the Cape, the Khoisan, is considerably lighter than that of either study group and may represent a long-established adaptation to seasonal UVB,” the researchers write.
“The darker skin of both study populations—Xhosia and Cape mixed—denotes a degree of mismatch between skin pigmentation and environmental UVB resulting from their recent migration into the region.”
The researchers found that both groups exhibited vitamin D deficiency during the winter, with women in both groups being more deficient, on average, than the men. Because of vitamin D’s impact on the immune system, the researchers provided six weeks of supplemental vitamin D3 to 30 of the Xhosa participants, which brought 77 percent of the participants to optimal vitamin D status.
Diet not a factor
Diet, genetics, and other variables played very small roles in vitamin D status, although some genetic variations did influence the success of supplementation.
To test how vitamin D status affected the immune system and HIV-1 in particular, the researchers exposed blood samples from Xhosa and Cape mixed participants taken during the summer and winter when the subjects were vitamin D sufficient or deficient.
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After nine days, the winter blood samples had greater infection than those taken in summer. After six weeks of vitamin D supplementation, the Xhosa blood sample levels of HIV-1 infection were the same as those during the summer.
“High-dosage oral vitamin D3 supplementation attenuated HIV-1 replication, increased circulating white blood cells, and reversed winter-associated anemia,” the researchers report. “Vitamin D3 presents a low-cost supplementation to improve HIV-associated immunity.”
Other researchers from Penn State and from University of Cape Town and Stellenbosch University contributed to the study.
A John Guggenheim Fellowship; The Stellenbosch Institute for Advanced Study; the Academy of Science of South Africa; South African Sugar Association; UK Medical Research Council; European Union 7th Framework Programme for Research and Technological Development; and Wellcome Trust supported the work.
Source: Penn State
