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"I think it’s one of the first, if not the first, times that a receptor switch of this nature has been identified," says Walter Atwood. (Credit: Wikimedia Commons)

viruses

Tiny mutation gives virus new target

The smallest mutation can completely change the target a virus will bind to on a victim cell—and possibly shift what kind of cell and eventually what kind of organism a virus can infect.

A new study shows that simply swapping a single amino acid can change the sugar that the human BK polyomavirus binds to on the surface of cells. Doing so makes the virus lose its ability to bind to its usual target sugar and instead “prefer” the same sugar as its cousin SV40 polyomavirus, which is active in monkeys.

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Researchers were working in cell cultures with safe pseudoviruses, which cannot spread, so they did not show that the pseudovirus changed its infectivity from one species to another, but the finding provides a new demonstration of how easily the binding target of a virus can change as its structure mutates and evolves.

Different cells have different binding targets on their surfaces. A change in a virus’s binding target preference can be a key step in changing how that virus would affect different cells in a victim—or move on to a different species.

“I think it’s one of the first, if not the first, times that a receptor switch of this nature has been identified,” says Walter Atwood, a virologist at Brown University and a corresponding author of the paper published in PLOS Pathogens.

“There are dozens of viruses that use these kinds of sugars as receptors. What we’re showing is that it doesn’t take much to convert from using one type of sugar to using another type of sugar. It helps us to understand evolutionarily how these viruses may adapt to a new host.”

The team learned of the single amino acid difference by comparing high-resolution structural models of the two polyomaviruses bound to their favorite sugars, says postdoctoral researcher Stacy-ann Allen, one of two lead authors on the paper.

Collaborators, including co-lead author Ursula Neu and co-correspondng author Thilo Stehle at the University of Tübingen in Germany, produced those models using nuclear magnetic resonance spectroscopy.

Virus makes the switch

“We had the structures and sequences of both BK and SV40, and they are relatively similar in their amino acid identity,” Allen says. “So when you see minute differences between them, you can target these differences to ask whether this difference allows for different infection in different hosts.”

Sure enough, when Allen made the change at amino acid site 68 in the BK polyomavirus, it switched from binding the “ganglioside” sugar GD3 to binding with GM1.

The researchers tested this not only in cells in the lab, but also by dropping the viruses onto microarrays of binding target sugars. And for even more confirmation, they sent the mutated BK viruses back to Germany for more NMR resolution.

“The NMR spectrum of the BK and the SV40 were identical,” Atwood says, “They thought they had mixed up the samples. They were identical in terms of their ability to bind to GM1, the monkey receptor.”

It may take several steps beyond a switch of receptor preference for a virus to infect new cells in the body or entirely new species, but such a switch could be a key step in more viruses than just the polyomavirus family, the scientists say. Others seem to switch preferences fairly quickly.

“Prominent examples include different serotypes of adenoviruses, the canine and feline paroviruses, as well as avian, swine, and human influenza viruses,” the authors write.

Researchers from Imperial College London, the University of Lübeck in Germany, and Biognos in Sweden contributed to the study, which was funded by the National Institutes of Health, the Wellcome Trust, and the UK Research Council.

Source: Brown University

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