PENN STATE (US)—An international team of scientists has completed sequencing and analysis of the genome for the Criollo variety of the cacao tree, generally considered to produce the world’s finest chocolate.
The analysis identifies a variety of gene families that may have future impact on improving cacao trees and fruit either by enhancing their attributes or providing protection from fungal diseases and insects.
“The large amount of information generated by this project dramatically changes the status of this tropical plant and its potential interest for the scientific community,” says Mark Guiltinan, professor of plant molecular biology at Penn State.
The researchers not only sequenced the genome of the ancient plant, but assembled 76 percent of the genome linking 82 percent of those genes to the 10 cacao chromosomes.
Details appear in Nature Precedings.
“Relics of the ancestral Criollo first cultivated by Olmec or Maya people can still be encountered in old Mesoamerican plantations or in forests where the Maya live,” says Siela Maximova, associate professor of horticulture.
“Our genome sequence is derived form a Belizean Criollo plant collected in the Mayan mountains.”
Cocoa production began in Mesoamerica 3,000 years ago, and the Criollo variety was the first domesticated. Because these trees self-pollinate, they are generally highly homozygous—possessing two identical forms of each gene, making it a good choice for accurate genome assembly.
Although originally a new-world crop, cacao trees—scientifically designated Theobroma cocao—are now grown and cultivated in humid, tropical areas around the world. About 3.7 million tons of cocoa are produced annually worldwide and contribute greatly to the income of small farmers.
Because they are grown in the shade, cacao trees are also ideal for environmental preservation because they contribute to diversity and land rehabilitation.
“We believe that Theobroma cacao is the first early domesticated tropical tree fruit crop to be sequenced,” Guiltinan says. “Interestingly, only 20 percent of the genome was made up of transposable elements.”
Transposable elements are one of the natural pathways through which genetic sequences change. They do this by moving around the chromosomes, changing the order of the genetic material. Smaller amounts of transposons than average could lead to slower evolution of the chocolate plant.
Guiltinan and his colleagues are interested in specific gene families that could link to specific cocoa qualities or disease resistance. They hope that mapping these gene families will lead to a source of genes directly involved in variations in the plant that are useful for acceleration of plant breeding programs.
“We hope this achievement will encourage greater investment in research of Theobroma cacao, the ‘food of the Gods’ whose magic flavor has spread worldwide since the time of the Maya and Aztec civilizations, and whose continued study will benefit developing countries for which cocoa is of high economic importance,” Guiltinan says.
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