Stop superbugs by turning up the heat

U. MINNESOTA (US) — One effective way to fight the spread of antibiotic-resistant bacteria, known as “superbugs,” may be to treat municipal wastewater solids at higher temperatures.

Heating the solid waste to 130 degrees Fahrenheit (55 degrees Celsius) was particularly effective in eliminating the genes that confer antibiotic resistance. Details are reported in the journal Environmental Science & Technology.

“The current scientific paradigm is that antibiotic resistance is primarily caused by antibiotic use, which has led to initiatives to restrict antibiotic prescriptions and curtail antibiotic use in agriculture,” says study leader Timothy LaPara, civil engineering associate professor at the University of Minnesota.

“Our research is one of the first studies that considers a different approach to thwarting the spread of antibiotic resistance by looking at the treatment of municipal wastewater solids.”

Antibiotic resistant bacteria develop in the gastrointestinal tracts of people taking antibiotics. These bacteria are then shed during defecation, which is collected by the existing sewer infrastructure and passed through a municipal wastewater treatment facility. The majority of wastewater treatment plants incubate the solid waste, called sludge, in a “digester” that decomposes organic materials. Digesters are often operated at 95 to 98 degrees Fahrenheit (35 to 37 degrees Celsius).

“Many digesters are operated at our body temperature, which is perfect for resistant bacteria to survive and maybe even grow,” LaPara says.

Lab research by LaPara and his graduate student David Diehl shows that anaerobic digestion of municipal wastewater solids at high temperatures (as high as 130 degrees Fahrenheit or 55 degrees Celsius) is capable of destroying up to 99.9 percent of various genes that confer resistance in bacteria. In contrast, conventional anaerobic digestion (operated at 95 to 98 degrees Fahrenheit or about 37 degrees Celsius) demonstrated only a slight ability to eliminate the same set of genes.

“Our latest research suggests that high temperature anaerobic digestion offers a novel approach to slow the proliferation of antibiotic resistance.” LaPara says. “This new method could be used in combination with other actions, like limiting the use of antibiotics, to extend the lifespan of these precious drugs.”

LaPara also points out that raising the temperature of anaerobic digestion at wastewater treatment plants is not cost-prohibitive because the digesting bacteria produce methane gas that can be used to heat the reactor.

The Minnesota Environmental and Natural Resources Trust Fund financially supported LaPara’s recent research. LaPara has secured a grant from the National Science Foundation to continue his research examining other technologies to eliminate antibiotic-resistant bacteria in wastewater solids.

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