Growing up on the space shuttle weakens a key part of the immune system of fruit flies.
It’s well established that spaceflight affects immune responses, says Deborah Kimbrell, a researcher in the department of molecular and cellular biology at the University of California, Davis, who led a new study published in PLOS ONE.
Drosophila flies share many fundamentals of the immune system with mammals such as mice and humans.
Kimbrell and colleagues initiated the first study of Drosophila immunity and gravity, using first hypergravity (increased gravity) and then microgravity, the decreased gravity of spaceflight.
The flies, which take about 10 days to develop into adults, were sent into space as eggs on a 12-day mission aboard the Space Shuttle Discovery.
After they returned to Earth, researchers tested their responses to two different infections: a fungus that flies fight off through a pathway mediated by the Toll receptor and a bacterial infection that flies resist through a gene called Imd (“immune deficiency”).
Gravity and immune response
Both the Toll and Imd pathways have counterparts in humans and other mammals—the 2011 Nobel Prize in physiology and medicine was awarded for discovery of Toll receptor activation of innate immunity in flies and mammals.
While the response through the Imd pathway was robust, the Toll pathway was “non-functional” in space-raised flies, Kimbrell says.
In Earth-based experiments, when flies were tested in a centrifuge under hypergravity conditions, their resistance to the fungus was improved, suggesting that their Toll pathway was boosted.
However, for the mutant yuri gagarin, which lacks normal responses to gravitational fields, resistance was the same at normal and hypergravity, further demonstrating a link between gravity and the immune response.
Future spacecraft designed for long missions could already include centrifuges that crew could use to keep up bone and muscle mass: it turns out that this might also have a beneficial effect on astronauts’ immune systems, Kimbrell says.
How does microgravity affect the immune system? Researchers have two hypotheses in mind, which are testable both in humans and flies:
- The space flies also showed high expression of genes for heat-shock proteins, which are produced in response to physiological stress. Heat shock proteins bind directly with mammalian Toll receptors, and may also moderate Toll activation in Drosophila.
- Microgravity interferes with the behavior of proteins outside the cell—an area that is more important for Toll than for Imd signaling.
Researchers from Rice University, the University of Central Florida, and the University of Nevada, Las Vegas contributed to the study.
Source: UC Davis