Sleepy fibroblasts act wide awake

PRINCETON (US) — Even in “sleep” mode, fibroblast cells are actually working much harder than previously thought to fend off destructive chemicals.

The findings suggest that other cells may be similarly misunderstood and more important to the human body’s longevity than scientists have realized.

Found in connective tissue such as ligaments and tendons, fibroblasts—integral to the body’s immune response and for healing wounds—have traditionally been viewed by scientists as crucial but dull, quietly performing their essential function.

The study reveals that when they go to sleep they enter a phase researchers term “quiescence,” and are actually more lively than sluggish.

“We’ve realized that these sleepy cells are not sleepy at all,” says Hilary Coller, assistant professor of molecular biology at Princeton University.

The research is published in the journal PLoS Biology.

“The broader implications could be that other cell types may also be metabolically active during quiescence as well,” Coller says, noting there could be a whole host of cell types that are more important to their environment than is currently understood.

Cells are considered quiescent when they are not actively proliferating—neither producing DNA nor dividing to produce offspring.

But while they may not be propagating, quiescent fibroblast cells produce proteins that help replenish other cells lost to damage or age. On the other hand, if cells fail to become quiescent when they should, tumors can be produced.

In addition, quiescent fibroblasts self-destruct when they are prevented from undergoing a series of chemical reactions, which could suggest a possible strategy for decimating dormant tumor cells, Coller says.

Previous studies had shown that other kinds of quiescent cells, such as disease-fighting white blood cells or lymphocytes, eat less when they enter this phase.

However, the fibroblasts, that were collected from skin and connective tissue and grown in the laboratory, are high-energy, charged-up beings, even when quiescent.

They eat and excrete plenty, chopping up and reformulating proteins and fatty acids, and hurling them into the extracellular matrix, the biological glue that holds cells into tissues. They also rely on a series of chemical reactions that to help eliminate damage-inflicting atoms known as free radicals.

“Our quiescent fibroblasts seem to be very different from quiescent lymphocytes,” Coller says. “They have important functions for the body even when they are not actively proliferating.

“They have the key ability to secrete proteins into extracellular environments, the space between cells that must contain important components in order to give the body structure and function.”

Johanna Lemons, a graduate student in the Department of Chemistry and the first author on the paper, conducted her research by dividing her efforts between Coller’s laboratory, where she harvested the cells using classic tissue culture techniques, and that of Joshua Rabinowitz, associate professor of chemistry and another author on the paper.

In Rabinowitz’s lab, Lemons analyzed metabolic processes by simultaneously measuring the levels of more than 65 core metabolites that made up the organisms’ “metabolic network.”

A metabolite is a chemical involved in metabolism, the process by which an organism takes nutrients from the environment and converts them to energy and the molecular building blocks that cells use to grow. Amino acids, sugars, nucleotides, and vitamins are all metabolites.

To conduct the study, the team used a mass spectrometry-based method developed by Rabinowitz, a highly sensitive technique that identifies chemicals based on size and electrical charge. The researchers tracked how the concentrations of metabolites in fibroblasts changed over time.

The team discovered that quiescent fibroblasts underwent a particular transformative cascade of chemical reactions that had only been thought to be used by actively dividing cells. When the scientists shut down this path in quiescent fibroblasts, the cells self-destructed in a process known as apoptosis.

The results could suggest ways to attack tumors, the scientists say. Some of the cells within a tumor can enter a state in which they are not dividing. They can remain dormant for years and without warning start dividing. Such cells are particularly difficult to target with current cancer treatments, which hunt down dividing cells for destruction.

By shutting down the same path as that followed by the quiescent fibroblasts, Coller says, they may be able to kill nondividing cancer cells.

“This represents an important avenue for further investigation.”

The research was funded by the Rita Allen Foundation, the Cancer Institute of New Jersey, the New Jersey Commission on Cancer Research, the Johnson & Johnson Foundation and the National Science Foundation. The work also included funding from three institutes within the National Institutes of Health: the National Institute for Allergy and Infectious Disease, the National Institute for General Medical Sciences and the National Cancer Institute.

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