fertilizer

Silver nanoparticles may stress out plants

DUKE (US) — Even low doses of silver nanoparticles can negatively affect plants and microbes, a study shows.

These preliminary findings are important, researchers say, because little is known about the environmental effects of silver nanoparticles, which are found in textiles, clothing, children’s toys, and pacifiers, disinfectants, and toothpaste.

“No one really knows what the effects of these particles are in the environment,” says Benjamin Colman, a postdoctoral fellow in Duke University’s biology department.

“We’re trying to come up with the data that can be used to help regulators determine the risks to the environment from silver nanoparticle exposures,” Colman adds.

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Previous studies have involved high concentrations of the nanoparticles in a laboratory setting, which the researchers point out, doesn’t represent “real-world” conditions.

“Results from laboratory studies are difficult to extrapolate to ecosystems, where exposures likely will be at low concentrations and there is a diversity of organisms,” Colman says.

Silver nanoparticles are used in consumer products because they can kill bacteria, inhibiting unwanted odors. They work through a variety of mechanisms, including generating free radicals of oxygen, which can cause DNA damage to microbial membranes without harming human cells.

The main route by which these particles enter the environment is as a by-product of sewage treatment plants. The nanoparticles are too small to be filtered out, so they and other materials end up in the resulting wastewater treatment “sludge,” which is then spread on the land surface as a fertilizer.

For their studies, the researchers created mesocosms, which are small, man-made structures containing different plants and microorganisms meant to represent the environment.

They applied sludge with low doses of silver nanoparticles in some of the mesocosms, and then compared plants and microorganisms from treated and untreated mesocosms after 50 days.

The researchers found that one of the plants studied, a common annual grass known as Microstegium vimeneum, had 32 percent less biomass in the mesocosms treated with the nanoparticles.

Microbes were also affected by the nanoparticles, Colman says.

One enzyme associated with helping microbes deal with external stresses was 52 percent less active, while another enzyme that helps regulate processes within the cell was 27 percent less active. The overall biomass of the microbes was also 35 percent lower, he says.

The study appears in the journal PLOS ONE.

“Our field studies show adverse responses of plants and microorganisms following a single low dose of silver nanoparticles applied by a sewage biosolid,” Colman says. “An estimated 60 percent of the average 5.6 million tons of biosolids produced each year is applied to the land for various reasons, and this practice represents an important and understudied route of exposure of natural ecosystems to engineered nanoparticles.”

“Our results show that silver nanoparticles in the biosolids, added at concentrations that would be expected, caused ecosystem-level impacts,” Colman says. “Specifically, the nanoparticles led to an increase in nitrous oxide fluxes, changes in microbial community composition, biomass, and extracellular enzyme activity, as well as species-specific effects on the above-ground vegetation.”

The researchers plan to continue to study longer-term effects of silver nanoparticles and to examine another ubiquitous nanoparticle—titanium dioxide.

Study collaborators include researchers from Coe College, Virginia Tech University, Carnegie Mellon University, University of Kentucky, and Wuhan Botanical Garden, China.

Funding from the National Science Foundation and the Environmental Protection Agency helped support the work.

Source: Duke University

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