Signs of prenatal smoking in DNA

USC (US)—Children exposed to maternal smoking in the womb show specific changes in DNA patterns that may affect health outcomes later in life.

The study by researchers at the University of Southern California found that children exposed in the womb to maternal smoking had differences in DNA methylation, an epigenetic mechanism in which small chemical compounds are added to DNA. The findings provide researchers with valuable insight into a biological process that is not well understood.

“This study provides some of the first evidence that in utero environmental exposures such as tobacco smoke may be associated with epigenetic changes,” says Carrie Breton, one of the lead authors and an assistant professor in USC’s Keck School of Medicine. “This could open up a new way for researchers to investigate biological mechanisms that might explain known health effects associated with maternal smoking.”

Epigenetics is the study of how chemicals that attach to DNA can switch genes on and off, leading to differences in gene expression without changing the fundamental genetic information. While epigenetics has become a robust field in cancer research, little is currently known about how such changes may be tied to environmental exposures.

Prenatal exposure to smoke is associated with a number of health problems, including childhood asthma, cardiovascular disease and lower pulmonary function later in life.

“Moms should not be smoking during pregnancy,” says Linda Birnbaum, director of the National Institute of Environmental Health Sciences, a component of the National Institutes of Health that helped fund the USC study. “Maternal smoking during pregnancy is not only detrimental to the health of the mom and the newborn child, but research such as this suggests that it may impact the child into adulthood and possibly even future generations as well.”

The study drew upon data from the USC Children’s Health Study, a longitudinal study of respiratory health among children in 13 Southern California communities.

Researchers looked at a subset of about 300 children from the 5,341 kindergarten and first graders who were enrolled in 2002. Information on maternal smoking exposure was collected using a questionnaire.

The findings showed that prenatal tobacco smoke exposure was associated with detectable changes in DNA methylation patterns for two types of DNA methylation: global methylation and methylation in the promoter region of cancer or developmental related genes.

Children exposed to prenatal smoke had a significantly lower level of global methylation, which was measured using the DNA repetitive element AluYb8. In contrast, children exposed to prenatal smoke had significantly higher methylation levels in the promoters of the genes AXL and PTPRO. Gene expression of both genes previously has been demonstrated to be correlated with DNA methylation levels.

“During pregnancy, there is a natural process of methylation reprogramming where small chemical compounds are removed from the DNA bases and then reattached a short while later, so it’s reasonable to think that an environmental exposure could alter DNA methylation during this period,” according to coauthor Hyang-Min Byun.

“This research could be an important first step into understanding how what happens in utero is tied to health outcomes later in life and how we may be able to intervene in these developments on an epigenetic level,” Breton adds. “For instance, it’s possible that exposure to maternal smoking may cause epigenetic changes that make children more susceptible to developing asthma.”

The study was supported by the National Institute of Environmental Health Sciences, the Hastings Foundation, and by an American Society of Clinical Oncology Career Development Award in Geriatric Oncology. The study appears in the September issue of the American Journal of Respiratory and Critical Care Medicine.

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