When disease-causing bacteria invade disease-resistant rice, a small protein produced by the bacteria betrays the invader. When the rice plant recognizes the protein, it senses that a microbial attack is underway and mounts an immune response to fend off infection, researchers report.
Identification of the tiny protein, called RaxX, holds promise for developing more disease-resistant crop varieties and therapeutic treatments for blocking microbial infections in both plants and animals, say the researchers, who found particular satisfaction in this discovery, two years after retracting the announcement of a similar find.
Results of the new study appear in Science Advances.
Disease protection for our food
In this new study, researchers discovered that the RaxX protein was present in at least eight species of the disease-causing Xanthamonas bacteria that are known to attack rice—the staple food for half of the world’s population—as well as maize, cassava, sugar cane, tomatoes, peppers, wheat, alfalfa, onions, banana, and citrus.
“Our research team is delighted to announce the discovery of the RaxX protein, a new class of microbial signaling molecules,” says Pamela Ronald, a professor of plant pathology at University of California, Davis, who directed the study.
Ronald notes that her laboratory is currently investigating the role of RaxX during bacterial infection of rice in the absence of the immune receptor.
The researchers have noticed that RaxX closely resembles a class of plant signaling factors that promote growth and modulate the immune response. They suspect that the bacteria could be mimicking these natural plant-signaling factors to inhibit the plant immune response and thereby enhance the competitiveness of the bacteria.
In the long term, the researchers hope to use this information to develop new strategies to prevent infection in various crops.
Mistakes along the way
Publication of the new study is particularly poignant for Ronald and lead coauthors Rory Pruitt and Benjamin Schwessinger, because it brings the research team full circle in correcting unintentional errors that led the Ronald lab in 2009 to misidentify the protein now known to be RaxX.
Pruitt and Schwessinger both worked on the new study as postdoctoral scholars in the Ronald lab, and Schwessinger is now an independent research fellow at the Australian National University in Canberra, Australia.
Ronald’s laboratory has been studying rice genetics and disease resistance for more than two decades and in 1995 announced that a gene called Xa21 confers resistance to the bacterial blight pathogen. Bacterial blight, one of the worst bacterial plant diseases in the world, has been found in virtually every crop species including rice.
The discovery of Xa21 was widely acclaimed by the scientific community and sparked further research into other key parts of the disease-resistance puzzle. Researchers were confident that if Xa21 produced a “receptor” in the plant cell that was capable of recognizing and thwarting a bacterial invasion, there must be a complementary protein in the bacteria that triggered that immune response in the plant.
In 2009 the Ronald lab announced discovery of a bacterial protein called Ax21, which their research indicated was the protein that triggers the immune response by the Xa21 plant receptor. A second related study, based on identification of Ax21, was published in 2011.
Then in 2013, as researchers in the Ronald lab began repeating the earlier experiments in preparation for a new study, they discovered that a bacterial strain had been mislabeled in the previous work and that one of the tests used in the earlier study turned out to be quite variable. These errors had led to the misidentification of Ax21 as the bacterial protein that sparks an immune response by the Xa21 receptor in the plant cells.
After finding the errors, Ronald retracted two papers from her laboratory about this research, published in 2009 and 2011 in the journals PLOS ONE and Science, respectively. She chronicled the story of that process in a post on Scientific American’s “Food Matters” blog.
Ronald and her laboratory colleagues have prepared a new posting for the Scientific American blog, which tells the story of the new discovery and the closure it brings in setting the scientific record straight.
Collaborating with Ronald, Schwessinger and Pruitt on the new study were researchers from UC Davis; Lawrence Berkeley National Laboratory; University of Tübingen, Germany; University of Texas at Austin; UC Irvine; and the Council of Scientific and Industrial Research, India.
The US National Institutes of Health, the US Department of Energy, the European Molecular Biology Association, the Human Frontiers Science Program, the Council of Scientific and Industrial Research in India, the Welch Foundation, and Monsanto’s Beachell-Borlaug International Scholars Program supported the work.
Source: UC Davis