biomarkers

Nanomagnets may speed blood tests

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Researchers have developed a new method for identifying and sorting diseased cells in blood samples using magnetic nanoparticles. “Effective and efficient separation is very important when you’re looking for a needle in a haystack,” says Ayse Rezzan Kose, lead author of the study. “We’re hoping we can achieve an increase of several orders of magnitude in the sensitivity of existing detection technologies.”

YALE (US)—A team has developed a way to rapidly manipulate and sort different cells in the blood using liquids comprised of magnetic nanoparticles. The advance could dramatically improve the speed and sensitivity of tests used to detect cancer biomarkers, blood disorders, viruses, and other diseases.

Details of the work led by Yale University scientists were published in the online edition of the Proceedings of the National Academy of Sciences.

Ferrofluids suspended throughout a liquid carrier, and have been used in industrial applications for years, including in hard disk drives and loudspeakers.

Now a team led by Hur Koser, associate professor at the Yale School of Engineering and Applied Science, has developed a biocompatible ferrofluid—one with the right pH level and salinity so that human cells can survive in it for several hours—and has created a device with integrated electrodes that generate a magnetic field pattern, allowing them to manipulate and separate red blood cells, sickle cells, and bacteria contained in this unique solution.

The magnetic field attracts the nanoparticles in the ferrofluid, effectively pushing and shuffling the much larger, nonmagnetic cells along specific channels. Depending on the frequency of the magnetic field they apply, the researchers are also able to manipulate and sort different types of cells depending on their size, elasticity, and shape.

Watch microparticles being directed along specific channels in the ferrofluid.

“It’s like the cells are surfing on magnetic forces,” Koser says. “When we turn on the magnetic field, the nonmagnetic cells are pushed immediately up to the top of the channel.” There, they roll along the surface and can be quickly directed toward a sensor.

While other cell manipulation techniques exist, this new method is unique in that it doesn’t require attaching biomarkers, or labels, to the cells and there is no need for labor-intensive preparation or post-processing.

Being able to effectively sort and move cells with this technique could allow for much greater efficiency in disease detection by directing diseased cells toward sensors. Many of today’s tests require hours or even days to complete, because the concentration of diseased cells in a blood sample may be so low that it takes a long time for them to randomly bump into the sensors. In early-stage cancer, for instance, there could be one tumor cell for every billion healthy cells, making them extremely difficult to detect.

“Effective and efficient separation is very important when you’re looking for a needle in a haystack,” says Ayse Rezzan Kose, a graduate student in the Koser Lab and the lead author of the study. “We’re hoping we can achieve an increase of several orders of magnitude in the sensitivity of existing detection technologies. If so, a blood sample analysis could be completed in minutes, not hours or days.”

Koser hopes that one day the new technique will lead to portable sensors that doctors can carry into the field and which could be used to test for a range of disorders, such as cancer and HIV. “Anything you can put into the ferrofluid solution is potentially detectable in this manner.”

Researchers from Yale and the University of Georgia contributed to the research that was funded by the National Science Foundation, the National Institutes of Health, and the Yale Institute for Nanoscience and Quantum Engineering.

Yale University news: www.opa.yale.edu/

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