NYU / PRINCETON (US)—Bacterial spores, the most resistant organisms on earth, carry an extra coating of protection previously undetected. The finding could shed light on why spores of the bacteria that cause botulism, tetanus, and anthrax survive methods to eradicate them.
Researchers from New York University and colleagues at Princeton University and Loyola University studied the spores of a non-pathogenic bacterium, Bacillus subtilis, which is commonly found in soil. B. subtilis spores exhibit many of the same structural features of the spore-forming pathogens.
The microbiologists examined the proteins that comprise spores’ protective layers. Previous research has shown that 70 different proteins make up these layers. Less understood is how these proteins interact to form the spores’ protective coats.
To do this, the researchers examined coat formation of both normal and mutant spores. In the latter case, they removed genes for selected coat proteins, allowing them to determine which proteins were necessary in—and extraneous to—the formation of the spores’ coats. Details of the findings are reported in the latest issue of the journal Current Biology.
To observe proteins’ behavior in living cells, the researchers fused the genes encoding the spores’ coat proteins to a marker, a green fluorescent protein (GFP). This procedure allowed them to monitor how the proteins localized to form spores’ protective coats.
A combination of fluorescence microscopy experiments and high-resolution image analysis enabled the researchers to overcome a theoretical limitation of light microscopy, pinpoint the location of the spores’ coat proteins with a high degree of precision, and build a map of the spore coat.
These experiments suggested the existence of a new outermost layer of the spore coat. They were then able to confirm the existence of this new layer using electron microscopy.
The researchers named this coat layer, located on the spores’ outer surface, the “spore crust.” While it has not yet been confirmed, it is possible that the spore crust is a common feature of all spore-forming bacteria, such as the botulism, tetanus, and anthrax pathogens.
Researchers from New York University, Princeton University, and Loyola University Medical Center contributed to the work, which was funded by the National Institute of General Medical Sciences, part of the National Institutes of Health.
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