PURDUE (US) — A new imaging tool that tracks carbon nanotubes in living cells and the bloodstream could advance their use for biomedical research and clinical medicine.
The structures have potential applications in drug delivery to treat diseases and imaging for cancer research. Two types of nanotubes are created in the manufacturing process: metallic and semiconducting. Until now, however, there has been no technique to see both types in living cells and the bloodstream.
The imaging technique, called transient absorption, uses a pulsing near-infrared laser to deposit energy into the nanotubes, which are then probed by a second near-infrared laser. The technique is called “label free,” because it doesn’t require that the nanotubes be marked with dyes, making it potentially practical for research and medicine.
The discovery overcomes key obstacles in using the imaging technology, detecting and monitoring the nanotubes in live cells and laboratory mice, says Ji-Xin Cheng, an associate professor of biomedical engineering and chemistry at Purdue University.
“Because we can do this at high speed, we can see what’s happening in real time as the nanotubes are circulating in the bloodstream.”
“It’s a fundamental tool for research that will provide information for the scientific community to learn how to perfect the use of nanotubes for biomedical and clinical applications,” he says.
The conventional imaging method uses luminescence, which is limited because it detects the semiconducting nanotubes but not the metallic ones.
The nanotubes have a diameter of about 1 nanometer, or roughly the length of 10 hydrogen atoms strung together, making them far too small to be seen with a conventional light microscope. One challenge in using the transient absorption imaging system for living cells was to eliminate the interference caused by the background glow of red blood cells, which is brighter than the nanotubes.
As reported in the journal Nature Nanotechnology, the researchers solved this problem by separating the signals from red blood cells and nanotubes in two separate “channels.” Light from the red blood cells is slightly delayed compared to light emitted by the nanotubes. The two types of signals are “phase separated” by restricting them to different channels based on this delay.
Researchers used the technique to see nanotubes circulating in the blood vessels of mice earlobes.
“This is important for drug delivery because you want to know how long nanotubes remain in blood vessels after they are injected,” Cheng says. “So you need to visualize them in real time circulating in the bloodstream.”
The structures, called single-wall carbon nanotubes, are formed by rolling up a one-atom-thick layer of graphite called graphene. The nanotubes are inherently hydrophobic, so some of the nanotubes used in the study were coated with DNA to make them water-soluble, which is required for them to be transported in the bloodstream and into cells.
The researchers also have taken images of nanotubes in the liver and other organs to study their distribution in mice, and they are using the imaging technique to study other nanomaterials such as graphene.
The research is funded by the National Science Foundation.
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