EMORY (US) — Using X-rays, researchers have identified the structure of a key protein from Lassa virus, which infects 100,000 to 300,000 people every year in West Africa—and kills 5,000.
The structure reveals how the virus evades its host’s immune system, and how it hijacks infected cells’ vital machinery in a process scientists call “cap-stealing.” Details of the structure, reported in the journal Nature, could guide future efforts at antiviral drug discovery and vaccine development.
Lassa virus represents a family of viruses—arenaviruses—whose natural hosts are rodents and cause hemorrhagic fevers in Africa and South America. New varieties of arenavirus continue to emerge, such as the deadly “Lujo” virus identified recently in Zambia and South Africa.
Most people infected have a mild illness, but about 20 percent have a severe multisystem disease with internal bleeding and immune suppression. Around one percent of all infections are fatal. A common complication of infection is deafness.
Lassa virus’ NP (nucleoprotein) is the first protein produced after viral infection and the most abundant component of the virus. To study the properties of Lassa virus NP, virologists at Emory University collaborated with structural biologists at University of St. Andrews, who examine the details of protein structure with X-rays. The X-ray data was collected at the Diamond Light Source, the United Kingdom’s national synchotron facility.
Human cells can usually detect viral infection with internal alarm systems that sense the presence of viral components, such as viral RNA or DNA. Arenaviruses such as Lassa virus have genomes made of RNA instead of DNA. Because cells send out molecules called interferons to muster the immune system against the virus, many viruses have developed ways to block interferon production.
Based on the X-ray structure, the authors discovered that Lassa virus NP has the ability to chew up RNA molecules. By testing versions of Lassa virus NP with that part of the protein disabled, they confirmed that this property is needed to suppress cells’ interferon production.
“What I think is exciting is that we are seeing a new viral mechanism for suppressing the interferon response,” says study senior coauthor Yuying Liang, assistant professor of pathology at Emory. “This is the first viral protein anyone has found that destroys the RNA molecules that are the triggers for these internal alarms.”
What remains a puzzle is how the virus avoids chewing up cellular and viral RNA indiscriminately, she says.
The researchers observed that Lassa NP can form rings by clustering in groups of three. They hypothesize that in a complete virus, the RNA genome is threaded through the three-part ring, with the RNA-chewing region of the protein facing out.
They also discovered that a separate region of Lassa virus NP can grip a cap structure normally found on RNA molecules produced by the host cell. This cap must be present at the beginning of all messenger RNAs to tell the cell’s protein-production machinery where to start.
“Lassa virus can’t make its own cap, so it steals one from the host,” says Hinh Ly, assistant professor of pathology at Emory and a senior coauthor of the study. “Seeing how a virus with only four genes can trick the cell and cause so many problems is a great illustration of efficiency. Each viral gene has to serve multiple functions.”
Influenza virus also performs the feat of cap-stealing, but the influenza virus protein that binds the cap structure does not look like the Lassa virus protein and the cap structure stolen by the flu virus differs from that of Lassa, he says.
Having structural information on Lassa virus NP may help scientists look for antiviral drugs. The only drug available against Lassa virus is ribavirin. Scientists say that how ribavirin works is unclear, and that it is only effective when used early, a significant limitation when Lassa virus infections often take a couple of weeks to become serious.
In Emory laboratories, researchers study individual genes and proteins from Lassa virus, but not the intact virus, because it is classified at the highest level of biosafety concern: BSL-4.
The research was supported by the Wellcome Trust, the (U.S.) National Institutes of Health, Southeast Regional Center of Excellence for Emerging Infections and Biodefense, and American Cancer Society.
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