This bodily fluid could provide new biomarkers

Microneedle patches containing micron-scale needles create temporary pores in the skin through which interstitial fluid can be extracted. (Credit: Allison Carter/Georgia Tech)

A minimally invasive technique for sampling a largely unexplored bodily fluid called dermal interstitial fluid uses an array of tiny needles.

Biochemical information about the body most commonly comes from analysis of blood—which represents only 6% of bodily fluids—but valuable information may also be found in other bodily fluids that are traditionally hard to get.

Researchers have now developed a way to extract dermal interstitial fluid (ISF), which circulates between cells in bodily tissues, using a simple through-the-skin technique that could provide a new approach for studying the metabolic products of cells, obtaining diagnostic biomarkers, and identifying potential toxins absorbed through the skin.

two tiny vials on palm of gloved hand
Interstitial fluid (right) may provide an alternative source of biomarkers compared to blood (left) that could be useful in diagnosing human illness. (Credit: Allison Carter/Georgia Tech)

Because the dermal interstitial fluid doesn’t clot like blood, the microneedle-based extraction could offer a new approach for continuous monitoring of glucose and other key health indicators.

Results of a human trial on the microneedle-based ISF sampling appear in the journal Science Translational Medicine.

“Interstitial fluid originates in the blood and then leaks out of capillaries to bring nutrients to cells in the body’s tissues. Because interstitial fluid is in direct communication with the cells, it should have information about the tissues themselves beyond what can be measured from testing the blood,” says Mark Prausnitz, professor and chair in Georgia Institute of Technology’s School of Chemical and Biomolecular Engineering.

“This microneedle-based technique could provide a minimally invasive and simple way to access this interstitial fluid to make it available for medical diagnostic and research applications.”

Pores get to the interstitial fluid

ISF has been difficult to sample. Indwelling instruments for monitoring glucose in ISF already exist, and other researchers have used surgically implanted tubing and vacuum-created blisters to extract ISF through the skin, but these techniques are not suitable for routine clinical diagnostic use.

The researchers used a patch containing five solid stainless steel microneedles that were a hundredth of an inch in length. By pressing the patch at an angle into the skin of 50 human subjects, they created shallow micropores that reached only into the outer layer of skin containing ISF. The researchers then applied a suction to the area of skin containing the pores and obtained enough ISF to do three types of analysis. For comparison, they also took blood samples and obtained ISF using the older blister technique.

To accurately determine the biomarkers available in the ISF, the researchers needed to avoid getting blood mixed with the ISF. Though major blood vessels don’t exist in the outer layers of skin, capillaries there can be damaged by the insertion of the microneedles. In their studies, the researchers found that if they slowly ramped up the suction after inserting the microneedles, they could obtain fluid clear of blood.

The overall extraction procedure took about 20 minutes for each test subject. The procedure was well tolerated by the volunteers, and the microscopic pores healed quickly within a day, with minimal irritation.

10,000 unique compounds

Researchers at Emory University analyzed the extracted fluid using liquid chromatography-mass spectrometry techniques to identify the chemical species it contained. Overall, there were about 10,000 unique compounds, most of which were also found in the blood samples. However, about 12% of the chemical species were not found in the blood, and others were found in the ISF at higher levels than in the blood.

“The skin is metabolically active, and it is full of cells that are changing the fluid,” Prausnitz says. “We found that some of the compounds were unique to the ISF, or enriched there, and that is what we were hoping to find.”

While not all the compounds unique to the ISF could be analyzed, the research team identified components of products that are applied to the skin—such as hand lotions—and pesticides that may enter the body through the skin. This discovery could set the stage for use of the microneedle technique for dermatological and toxicology studies.

“If you want to look at what accumulates in the skin over time, this may provide a way to obtain information about those kinds of exposures,” Prausnitz says. “These are materials that may accumulate in the tissues of our body but are not found in the bloodstream.”

Glucose in the blood and ISF

The researchers also determined the pharmacokinetics of caffeine and the pharmacodynamics of glucose—both small molecules—from the ISF, indicating that that dynamic biomarker information could be obtained from the technique. Those measurements suggested that ISF could provide a means for continuously monitoring such compounds, taking advantage of the fact that the fluid does not clot.

“We were encouraged that we found a good correlation between the blood and interstitial fluid glucose, which suggests we might be able to have a continuous glucose monitoring system based on this technology,” Prausnitz says. A microneedle-based system could provide a less invasive alternative to existing implantable glucose sensors by allowing the sensing components to remain on the surface of the skin.

In future research, Prausnitz would like to reduce the time required to extract the ISF and simplify the process by eliminating the vacuum pump. Additional study of the compounds found in the fluid could also show whether they have medical diagnostic value.

“We’d like to make this microneedle-based technique available to the research community to make ISF routinely available for study,” he says. “Tissue interstitial fluid could be a novel source of biomarkers that complements conventional sources. This research provides a means to study this further.”

Support for the work came from the National Institutes of Health and Children’s Healthcare of Atlanta. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the funding agencies.

Prausnitz is an inventor of patents licensed to companies developing microneedle-based products, is a paid advisor to companies developing microneedle-based products, and is a founder/shareholder of companies developing microneedle-based products (Micron Biomedical). This potential conflict of interest has been disclosed and is managed by Georgia Tech. First author of the paper Pradnya P. Samant and Prausnitz are inventors on a patent application submitted by Georgia Tech Research Corporation that covers ISF collection methods presented in this study.

Source: Georgia Tech