Immunity gene shields frogs from fungus

CORNELL (US) — A genetic mechanism in lowland leopard frogs makes them resistant to a deadly fungus that has been decimating other frog species for decades.

Although past research has explored environmental and pathogenic factors that contribute to the often fatal chytridiomycosis, a new study, published in Proceedings of the National Academy of Sciences, looks specifically at host genetic factors that might play a role in resistance to the pathogen Batrachochytrium dendrobatidis (Bd).

Lowland leopard frog killed by the fungal disease chytridiomycosis. (Credit: Anna Savage)

Scientists discovered that variation in a gene associated with a frog’s ability to identify pathogens and initiate an immune response determined whether a frog resisted the disease and also found evidence that one form of the gene that gives frogs immunity has been positively selected in recent generations.

The findings offer hope that frogs may adapt to the disease, as long as their habitats are protected and their populations expand enough to diversify their gene pools.

“This is the first demonstration that host genetics determine susceptibility to Bd,” says Anna Savage, lead author of the paper and a graduate student working in the lab of lead author Kelly Zamudio, professor of ecology and evolutionary biology at Cornell University.

Savage reared lowland leopard frogs from five distinct populations in Arizona to a disease-free adulthood in the lab. She then infected them with a Bd strain that was new to all five populations. All the frogs from three populations died. In the two remaining populations, seven frogs from each survived.

Savage then analyzed immune system major histocompatibility complex (MHC) genes, which code for a molecule that binds to foreign pathogens and initiates an immune response in the host. Specifically, she sequenced MHC genes that control the regions of the molecules that bind, like a lock and key, to pathogens. If the molecule and Bd bind, the frog survives.

Savage found 33 distinct alleles (or forms of this MHC gene), showing large variability. Almost all of the frogs that had two forms of the gene (called heterozygotes) survived, while almost all of the frogs with only one form (homozygotes) died.

Since Bd has many proteins that could be recognized by different MHC molecules, having more than one form of the MHC gene may have increased the survivors’ chances for binding to the pathogen. The researchers also found that one of the 33 gene variants, called allele Q, was only found among survivors.

“The study shows that allele Q is a candidate resistance allele, and more broadly, heterozygous frogs had a higher chance of survival,” Savage says. She also found evidence for positive selection along the evolutionary lineage leading to allele Q, which provides hope that frogs will evolve and adapt to Bd if habitats are maintained.

“This is one case where we have shown selection and adaptation for resistance to this particular disease,” says Zamudio. “The hope is that we can detect this signal of evolved resistance to other species as well.”

The study was funded in part by the National Science Foundation.

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