PURDUE (US) — Seeing the mosquito-transmitted chikungunya virus pathogen at very high resolution while it’s bound to antibodies could lead to vaccines for the disease.
The infection causes symptoms similar to dengue fever, followed by a prolonged disease that affects the joints and causes severe arthritis. In recent outbreaks, some cases progressed to fatal encephalitis.
The researchers studied “virus-like particles,” or non-infectious forms of the virus. They also obtained near atomic-scale resolution of the virus attached to four separate antibodies.
“We knew these antibodies neutralize the real virus, so we wanted to know how they do it,” says Michael Rossmann, professor of biological sciences at Purdue University.
As reported in the journal eLife, the scientists used a technique called cryoelectron microscopy to uncover critical structural details about the virus-like particles bound to the antibodies. The particles are made of 180 “heterodimers,” molecules made of two proteins: envelope protein 1, or E1, and envelope protein 2, or E2.
The findings show the precise structure of the virus-like particle bound to a key part of the antibodies, called the antigen binding fragment, or Fab, which attaches to the heterodimers making up the virus’s outer shell. The analyses showed that the antibodies stabilize the viral surface, hindering fusion to the host cell and likely neutralizing infection.
Chikungunya is an alphavirus, a family of viruses that includes eastern equine encephalitis. “This is the first time the structure of an alphavirus has been examined in this detail,” Rossmann says.
The research team conducted experiments to record the structure of the virus in different orientations and obtained a three-dimensional structure with a resolution of 5.3 Ångstroms, or 5.3 ten-billionths of a meter.
The research is aimed at learning precisely how viruses infect humans and other hosts, knowledge that may lead to better vaccines and antiviral drugs, Rossmann says.
In 2005, Chikungunya caused an epidemic on Réunion Island. A mutation in the E1 protein has allowed the virus to replicate more efficiently, which is considered the primary reason for its recent extensive spread, infecting millions of people in Africa and Asia.
Researchers from Purdue, the National Institutes of Health, and the Washington University School of Medicine are additional co-authors of the paper.
The research, funded by the NIH, is ongoing.
Source: Purdue University