Glowing virus IDs anthrax in hours

"In the years since the post 9/11 postal attacks, we haven't had any bona fide anthrax attacks," says George Stewart. "That doesn't mean that it's not going to happen, we just have to be prepared for when it does occur again." (Credit: University of Missouri)

Shortly following the 9/11 terror attack in 2001, letters containing anthrax spores were mailed to news outlets and government buildings killing five people and infecting 17 others. According to a 2012 report, the bioterrorism event cost $3.2 million in cleanup and decontamination.

[related]

At the time, there was no testing system for officials to use to screen the letters.

Currently, first responders have tests that can provide a screen for dangerous materials in about 24 to 48 hours.

Now, researchers at the University of Missouri have worked with a private company to develop a new method that can identify anthrax in only a few hours using a glowing virus.

“Normally to identify whether an organism is present, you have to extract the material, culture it, and then pick colonies to examine that might turn out to be anthrax bacteria,” says George Stewart, a medical bacteriologist the University of Missouri Bond Life Sciences Center and chair of the Department of Veterinary Pathobiology.

“Then you conduct chemical testing which takes some time—a minimum of 24 to 48 hours. Using this newly identified method, we can reduce that time to about five hours.” The team’s findings are published in the Journal of Microbiological Methods.

Glowing presence

Using a virus known as a “bioluminescent reporter phage,” Stewart and graduate student Krista Spreng tested the phage at the university’s Laboratory for Infectious Disease Research.

The phage, developed by David Schofield at Guild BioSciences, a biotech company in Charleston, SC, is injected in the sample causing anthrax to glow if present.

The team also found that the method can detect low levels of anthrax bacteria and rule out false positives. The added benefit to this reporting system is its ability to show if anthrax is present and whether or not the spores are alive, Stewart says.

The next step for the research team will be to get the bioluminescent reporter phage approved by federal regulatory agencies so a product can be produced and distributed, Stewart says.

“In the years since the post 9/11 postal attacks, we haven’t had any bona fide anthrax attacks,” Stewart says. “That doesn’t mean that it’s not going to happen, we just have to be prepared for when it does occur again.”

The United States Department of Agriculture funded the research.

Source: University of Missouri