CALTECH (US) — To learn more about human cell migration works—and goes awry—biologists have turned to a tiny roundworm.
Despite their small size, the worms (Caenorhabditis elegans) actually share quite a few genes with humans.
“Migration is such a conserved process,” says Mihoko Kato, a senior research fellow in biology at the California Institute of Technology (Caltech) and a co-author of a paper published in the Proceedings of the National Academy of Sciences. “So whether it happens in C. elegans or in mammals, like humans, we think that many of the same genes are going to be involved.”
At any given moment, millions of cells are on the move in the human body, typically on their way to aid in immune response, make repairs, or provide some other benefit to the structures around them.
When the migration process goes wrong, however, the results can include tumor formation and metastatic cancer. Little has been known about how cell migration actually works.
“In terms of cancer, we know how to find primary tumors and we know when they’re metastatic, but we’re missing information on the period in between when cells are crawling around, hanging out, and doing who knows what that leads to both of these types of diseases,” says Paul Sternberg, a biology professor at Caltech and corresponding author of the paper.
Contained in each cell—be it human or worm—are thousands of genes, all of which have a special job, or jobs, to do. Of these genes, roughly one-third are active in a given cell.
To see what genes are expressed during migration, Sternberg and Kato, along with Erich Schwarz, a research fellow in Sternberg’s lab, studied a single cell, called the linker cell (LC), in the worms; during reproductive development, LCs travel almost the entire length of the worm’s body.
Using high-powered microscopy, the team identified LCs at two intervals, 12 hours apart, during the worm’s larval stage, and removed them from the animals.
Then, using sequencing and computational analysis, they determined the genes that were actively expressed at these two migration time points. This method of study is called transcriptional profiling.
“By understanding the normal migration of a single cell, we can understand something about how the cells are programmed to navigate their environment,” says Sternberg, who is also an investigator with the Howard Hughes Medical Institute.
“Our view of cancer metastasis is that the tumor cells confront some obstacle and then they have to evolve to get through or around that obstacle. The way they probably do that is by using some aspect of the normal program that exists somewhere in the genome.”
He says that learning more about different ways that cells migrate may lead to the development of new types of drugs that block this process by targeting specific genes. The team plans additional transcriptional profiling studies to obtain more detailed information about the functions of particular C. elegans genes involved in migration—and, eventually, of similar genes in higher organisms, including humans.
“We selected genes present in both worms and humans, but which have not been studied much before us,” says Schwarz. “Since we found that some of these genes help worm LCs migrate, we think each one may have a related human gene helping cells migrate, too.”
“The nice thing about this technology is that you can use it with any cell type,” adds Kato, who points out that their studies have already helped identify new functions for known genes possessed by both the worms and humans. “It’s a similar process to do transcriptome profiling using human cells.”
In addition to identifying drug targets, the team is also hoping to find a good signature, or molecular marker, for migrating cells.
“This kind of information could be very useful diagnostically, to help identify cells that are doing things that they shouldn’t be doing, or weird combinations of genes that shouldn’t be expressed together, which is what a tumor cell might have,” says Sternberg. “This work lays the foundation for really understanding what information is critically needed from mammalian cells for tumor cells to be able to migrate.”
The National Institutes of Health and the Howard Hughes Medical Institute funded the work.