Researchers have figured out how to objectively quantify the beating action of cilia, the tiny, hair-like projections on cells that line nasal passages, the lungs, and almost every other body tissue.
Such digital signatures could help doctors more quickly and accurately diagnose ciliary motion (CM) defects, which can cause severe respiratory airway clearance defects and also developmental defects including congenital heart disease.
Currently, doctors try to identify CM defects using video-microscopy or indirectly via the examination of cilia ultrastructural defects using electron microscopy. This usually entails analysis of cilia movement in respiratory cells obtained from nasal passages, explained senior investigator Chakra Chennubhotla, assistant professor of computational and systems biology at the University of Pittsburgh School of Medicine.
“Visual reviews like these can be subjective, time-consuming, and error-prone,” he says. “In this project, our team used computational methods to objectively and reliably identify CM defects.”
The researchers used two independent data sets—one from Children’s Hospital of Pittsburgh of UPMC (CHP) and the other from Children’s National Medical Center (CNMC) in Washington, DC—from healthy individuals as well as patients already diagnosed with either congenital heart disease or primary ciliary dyskinesia (PCD) to identify the digital signatures of normal and abnormal movement, accounting for factors such as how frequently the cilia beat back and forth, the breadth and rotation of their beat pattern, and their synchronicity.
The researchers then validated their technique by testing the patient samples in blind fashion, finding that the computational tool correctly identified more than 90 percent of PCD cases at CHP and all of the cases at CNMC. PCD is a rare condition in which the cilia are immotile or beat abnormally, leading to limitation of airway mucus clearance, compromised respiratory function, and increased risk for lung infections and other bronchial problems.
“We hope to start a clinical trial in which doctors from around the country can upload a video of their patient’s nasal lining to a website for assessment of ciliary motion with this technique,” says co-investigator Cecilia Lo, professor and chair of developmental biology. “If successful, this approach may in the future serve as a rapid first-tier screen to identify at-risk patients.”
The National Institutes of Health and the Pennsylvania Department of Health supported the work, which appears in Science Translational Medicine.
Source: University of Pittsburgh
