Scientists have found the first actual “sensor mechanism” that allows a living cell to detect an electric field.
“We believe there are several types of sensing mechanisms, and none of them are known. We now provide experimental evidence to suggest one which has not been even hypothesized before, a two-molecule sensing mechanism,” says Min Zhao, a researchers at the UC Davis Institute for Regenerative Cures.
Zhao and colleagues have been studying these “electric senses” in cells from both larger animals (fish skin cells, human cell lines) and in the soil-dwelling amoeba Dictyostelium. By knocking out some genes in Dictyostelium, they previously identified some of the genes and proteins that allow the amoeba to move in a certain direction when exposed to an electric field.
In the new work, carried out in a human cell line, they found that two elements, a protein called Kir4.2 (made by gene KCNJ15) and molecules within the cell called polyamines, were needed for signaling to occur.
Kir4.2 is a potassium channel—it forms a pore through the cell membrane that allows potassium ions to enter the cell. Such ion channels are often involved in transmitting signals into cells. Polyamines are molecules within the cell that carry a positive charge.
They found that when the cells were in an electric field, the positively charged polyamines tend to accumulate at the side of the cell near the negative electrode. The polyamines bind to the Kir4.2 potassium channel, and regulate its activity.
Zhao cautions that they do not yet have definitive evidence of how “switching” of the potassium channel by polyamines translates into directional movement by the cell.
Other scientists from UC Davis, Zhejiang University in China, and the Institute of Molecular Biotechnology of the Austrian Academy of Sciences in Austria collaborated on the study, which appears in Nature Communications. Grants from the National Institutes of Health, NSF, American Cancer Society, American Heart Association, Research to Prevent Blindness Inc., and the University of California supported the project.
Source: UC Davis