A small change in a protein called PTBP1, which spurs the creation of neurons, may be the key to how mammalian brains evolved to be the largest and most complex among vertebrates.
Brain size and complexity vary enormously across vertebrates, but it is not clear how these differences came about. Humans and frogs, for example, have been evolving separately for 350 million years and have very different brain abilities. Yet scientists have shown that they use a remarkably similar repertoire of genes to build organs in the body.
So how is it that a similar number of genes, that are also switched on or off in similar ways in diverse vertebrate species, generate a vast range of organ size and complexity?
LEGO gene fragments
The key involves a process known as alternative splicing (AS), where gene products are assembled into proteins, which are the building blocks of life. During AS, gene fragments—called exons—are shuffled to make different protein shapes. It’s like LEGO, where some fragments can be missing from the final protein shape.
AS enables cells to make more than one protein from a single gene, so that the total number of different proteins in a cell greatly surpasses the number of available genes. A cell’s ability to regulate protein diversity at any given time reflects its ability to take on different roles in the body.
Previous work has shown that AS prevalence increases with vertebrate complexity. So although the genes that make bodies of vertebrates might be similar, the proteins they give rise to are far more diverse in animals such as mammals, than in birds and frogs.
And nowhere is AS more widespread than in the brain.
“We wanted to see if AS could drive morphological differences in the brains of different vertebrate species,” says Serge Gueroussov, a graduate student in the lab of Benjamin Blencowe, professor of medical research at the University of Toronto.
Gueroussov, the current study’s lead author, previously helped identify PTBP1 as a protein that takes on another form in mammals, in addition to the one common to all vertebrates. The second form of mammalian PTBP1 is shorter because a small fragment is omitted during AS and does not make it into the final protein shape.
Could this newly acquired, mammalian version of PTBP1 give clues to how our brains evolved?
PTBP1 is both a target and major regulator of AS. The protein’s job in a cell is to stop it from becoming a neuron by holding off AS of hundreds of other gene products.
The new research, published in the journal Science, shows that in mammalian cells, the presence of the second, shorter version of PTBP1 unleashes a cascade of AS events, tipping the scales of protein balance so that a cell becomes a neuron.
What’s more, when Gueroussov engineered chicken cells to make the shorter, mammalian-like PTBP1, this triggered AS events that are found in mammals.
“One interesting implication of our work is that this particular switch between the two versions of PTBP1 could have affected the timing of when neurons are made in the embryo in a way that creates differences in morphological complexity and brain size,” says Blencowe, who is also a professor of molecular genetics.
As scientists continue to sift through countless molecular events occurring in our cells, they’ll keep finding clues as to how our bodies and minds came to be.
“This is the tip of an iceberg in terms of the full repertoire of AS changes that likely have contributed major roles in driving evolutionary differences,” Blencowe says.
Source: University of Toronto