BROWN (US) — A new tool visually tracks the transformation of a living population of stem cells into cells of a specific tissue—in real time.
The molecular beacons light up when certain genes are expressed and don’t interfere with the development or operation of the stem cells. Tissue engineers say the technology could offer a potentially powerful way to discover what it takes to make stem cells transform into desired tissue cells more often and more quickly—a key goal in improving regenerative medicine treatments.
“We’re not the inventors of molecular beacons but we have used it in a way that hasn’t been done before, which is to do this in long-term culture and watch the same population change in a reliable and harmless way,” says graduate student Hetal Desai, lead author of a new paper published online in the journal Tissue Engineering Part A.
Desai and corresponding author Eric Darling, assistant professor of biology in the Department of Molecular Pharmacology, Physiology, and Biotechnology, designed the beacons to fluoresce when they bind to mRNA from three specific genes in fat-derived stem cells that are expressed only when the stem cells are transforming into bone cells.
Glowing green spots in these MG-63 bone cells (each blue dot is a nucleus) indicate that a fluorescent “beacon” molecule has bound to RNA produced by expression of the bone-specific ALPL gene. (Credit: Darling Lab/Brown University)
Throughout 21 days of their development, the cells in the experiments remained alive and unfettered, except that some populations received a chemical inducement toward becoming bone and others did not. Over those three weeks, the team watched the populations for the fluorescence of the beacons to see how many stem cells within each population were becoming bone and the timing of each gene expression milestone.
The beacons’ fluorescence made it easy to see a distinct pattern in that timing. Expression of the gene ALPL peaked first in more than 90 percent of induced stem cells on day four, followed by about 85 percent expressing the gene COL1A1 on day 14. The last few days of the experiments saw an unmistakably sharp rise in expression of the gene BGLAP in more than 80 percent of the induced stem cells.
Each successive episode of gene expression ramped up from zero to the peak more quickly, the researchers noted, leading to a new hypothesis that the pace of the stem cell transformation, or “differentiation” in stem cell parlance, may become more synchronized in a population over time.
“If you could find a way to get them on this track earlier, you could get the differentiation faster,” Darling says.
Meanwhile the stem cell populations that were not induced with bone-promoting chemicals, showed virtually no beacon fluorescence or expression of the genes, indicating that the beacons were truly indicators of steps along the transformation from stem cell to bone.
Beacons don’t affect cells
The team took extra care to design beacons that would not alter the cells’ development or functioning in any way. While the beacons do bind to messenger RNA produced in gene expression, for example, they do not require adding any genes to the stem cells’ DNA, or expressing any special proteins, as many other fluorescence techniques do.
The team performed several experiments using the beacons in conventionally developing bone cells to make sure that they developed normally even as the beacons were in operation. While some scientists design RNA-based probes to purposely interfere with gene expression, this team had the opposite intent. “There’s a set of rules for interference RNA, and we essentially did the opposite of what those rules said you should do,” Desai says.
Toward quicker healing
Now that the beacons’ performance in indicating milestones of stem cell differentiation has been demonstrated, the technology can be applied to studying the process in a wide variety of cells and under a variety of other experimental conditions.
In the case of tissue engineering, the beacons can aid experiments seeking to determine what conditions (inducing chemicals or otherwise) are most effective in converting the most stem cells to desired tissues most quickly. They could help tissue engineers learn the best timing for adding an inducing chemical. They might also provide a way for tissue engineers to identify and harvest only those cells that are converting to the desired tissue, Darling says.
“They are becoming bone cells and if you enrich for them and you get rid of all the ones that aren’t becoming bone cells, it stands to reason that you will have a better product at the end.”
More broadly, molecular beacons are proving useful in a wide variety of gene expression studies.
“The reason we are looking at this technique is that we wanted something we could use on any cell, look at any gene and not affect that cell while we are looking at it,” Darling says. “If this is acting as we believe it is, we can really look at any gene that we want. It seems like a very versatile tool.”
The National Institutes of Health provided funding for the research.
Source: Brown University