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Antigen discovery sets up salmonella vaccine

UC DAVIS (US) — Researchers have taken an important step toward an effective vaccine for the foodborne bacteria salmonella.

The research team has identified a set of antigens—molecules in the invading bacteria that trigger an immune response—that is common to both mice and humans. They report their findings in the journal Proceedings of the National Academy of Sciences.

Salmonella, a group of increasingly antibiotic-resistant foodborne bacteria, kills hundreds of thousands of people worldwide each year.

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“These antigens will provide the research community with a foundation for developing a protective salmonella vaccine,” says Stephen McSorley, an immunologist and associate professor in the University of California, Davis Center for Comparative Medicine, which investigates diseases that afflict both humans and animals.

Salmonella bacteria cause foodborne illness in industrialized nations. More than 1.4 million cases occur annually in the United States alone, according to the World Health Organization, at an estimated cost of $3 billion and the loss of 580 lives.

There are currently no vaccines for the strains of salmonella that cause these types of illnesses. Furthermore, salmonella bacteria increasingly are becoming resistant to existing antibiotic treatments. And no new, effective antibiotics are on the horizon.

“Although salmonella infections are extremely important to human and animal
health around the world, up until this time, the target antigens that are so key to developing a vaccine had not been clearly defined,” McSorley says.

In an effort to identify those antigens, the research team created an array, or collection, of 2,700 proteins, representing approximately 60 percent of all proteins produced by salmonella bacteria.

The researchers found that 117 of those proteins behaved as antigens when mixed with blood serum from salmonella-infected mice, triggering an immune response to defend against the bacterial infection. Fourteen of those proteins were common to all four strains of mice involved in the study.

The researchers also identified 14 proteins that served as antigens in the blood serum from Malawian children infected with salmonella. Eight of those 14 proteins, or 57 percent, were among the 117 antigens identified in the mice.

“Discovery of the eight antigens in both mouse and human infections suggests that some of these antigens might be successfully used in developing a vaccine to protect against salmonella and that the mouse model of salmonella will be useful before vaccine research moves into clinical trials,” McSorley says.

Additional researchers contributed from UC Irvine, the University of Malawi, Novartis Vaccines Institute for Global Health, and the University of Birmingham.

The National Institutes of Health, the Wellcome Trust, and GlaxoSmithKline provided funding for the study.

More news from UC Davis: www.news.ucdavis.edu/

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