Molecular Cell _1

Turning up volume on cellular noise

DUKE (US) — While some scientists find it messy that cells of the same type will respond differently to identical stimuli, bioengineers have now turned this cellular noise to their advantage.

In doing so, they have discovered important insights into cues that tell cells when to begin to make copies of themselves, a process known as the cell cycle.

The scientists at Duke University knew that the expression of a particular gene is responsible for launching the cell cycle and it has the capability to tell a cell to go into hibernation or kill itself, like a cellular dead-man’s switch.

This same gene is also known to be present in increased amounts in several types of human cancer.

Understanding what flips the switch could provide a new avenue for treating diseases marked by abnormal cell proliferation, such as cancer.

Using a commonly observed but often ignored phenomenon—cell-to-cell variability in virus infection—scientists delivered increasing amounts of the gene to a population of rat fibroblast cells growing in culture, and observed its effects on certain indicators of proliferation.

This variability of gene expression within a genetically identical population of cells is termed noise.

“Over the past 10 years, many studies have looked at this phenomenon of noise, and biologists have often found this variability to be messy,” says Lingchong You, assistant professor of biomedical engineering.

“Here, we took the opposite approach. When coupled with single-cell measurements and computer modeling, this variability provides an efficient means to measure and understand how levels of gene expression affect signaling dynamics in mammalian cells.”

Details of the experiments are published online in the journal Molecular Cell.

The gene in question is known as c-Myc. It is believed that this single gene participates in regulating as much as 15 percent of all human genes. When mutated, it has been implicated in many forms of cancers.

“The amount, or dosage, is key,” says Jeffrey Wong, post-doctoral fellow in You’s laboratory and the paper’s first author.

“We found that as the amount of c-Myc increased, the expression of an important protein also increased, but then subsequently decreased as the amount of c-MYC continued to increase.”

The protein is called E2F1, which is vital to the cell cycle process and can act as a tumor suppressor.

Under normal circumstances, low levels of c-Myc provide the trigger for the production of E2F1, which in turn tells the cell to start dividing.

Researchers introduced increasing amounts of c-Myc into rat cells growing in culture and found that as the amount reached higher levels, the cells’ first response was not to proliferate normally, but to remain quiescent instead.

“This response of normal cells to high levels of c-Myc is, depending on the type of cell, either to remain inactive or to commit suicide. It’s like a built-in safeguard against over-proliferation,” says You.

However, abnormal cells in tumors might find a way around this barrier. In these cases, amplification of c-Myc and additional mutations may cooperate to upset normal constraints on growth control, You said, which could be a key to the emergence of aggressive cancers.

The research was supported by the National Institutes of Health, a David and Lucille Packard Fellowship, and a DuPont Young Professorship.

More news from Duke University: www.dukenews.duke.edu

chat4 Comments

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4 Comments

  1. Roy Niles

    If each cell is “thinking” based on feeling and reacting, then the interaction will somewhat paradoxically require individual differences in responses. No?

  2. Jim Costopulos

    Roy, the research here is using the phenomenon of cell-to-cell variability to select for cells with differential expression of the target gene for the subsequent experimental work. The first sentence of the paper abstract states: “Gene expression mediated by viral vectors is subject to cell-to-cell variability, which limits the accuracy of gene delivery.” It is not at all hard to envision variability in subsequent gene expression after gene insertion by viral vectors originating within the mechanical, chemical, mutation or other processes that occur during gene transference. Further, in this research a successful predictive model was built to mirror the biphasic pattern of protein production resulting from the original gene variability (the initial “noise” phenomenon). I realize you may be speaking from a larger philosophical context of your own design, but there does not appear to be anything paradoxical here that should prompt an intelligent person to suggest cell-level “consciousness” as an explanation, for which there is no rational basis.

  3. Roy Niles

    Well, it’s not necessarily by my own design that others (Wetware: A Computer in Every Living Cell, etc.) have proposed that there are choice making functions at the cell level, and choices based on an awareness of options concomitant with an awareness of the differences in sensory input – all cells being different to some necessary degree. All consciousness necessarily (philosophically speaking) representing some degree of awareness.

  4. Roy Niles

    Except that I didn’t initially say “consciousness,” I said “thinking,” which does of course imply awareness, which Jim may not think exists at the cell level either, or that some cells may appear to think (e. coli?), but not those that are endemic to rats.

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