Seasonal depression linked to this brain region

"Before, we thought serotonin was probably involved. Now we know that serotonergic neurons are definitely involved," says Douglas McMahon. (Credit: Rob Baird/Flickr)

About 5 percent of Americans feel depressed during certain seasons, a condition known as seasonal affective disorder (SAD).

Scientists know exposure to sunlight and circadian clocks play a role, and they suspect the neurotransmitter serotonin and the hormone melatonin are involved.

New research with mice is helping to identify the underlying mechanisms. Biologists at Vanderbilt University say a small region in the mid-brain—the dorsal raphe nucleus—is the source of the seasonal light cycle effects that drive SAD.

In both mice and humans, the dorsal raphe nucleus is an area where many of the specialized neurons that control serotonin levels throughout the brain are located. Because high concentrations of serotonin are associated with feelings of well-being and happiness, while low levels are linked to depression, it plays a major role in regulating an individual’s mood.

Born in summer or winter?

Their study also found that the day/night cycle in which individuals are born can have a long-lasting effect on the activity level of the neurons in this region.

“We got the idea for the study from a report by Viennese psychiatrists, which found a season of birth correlation in SAD patients,” says Noah Green, a graduate student and the study’s lead author.

The biologists decided to concentrate their investigation on the dorsal raphe nucleus because prior studies had shown that it is linked to the brain’s master biological clock and it also responds to melatonin, a hormone that is involved in the regulation of a number of related physiological functions, including sleep, blood pressure, and seasonal reproduction.

To test the effect of seasonal light cycles, they divided mice into three groups. One group was born and raised in an environment with a summer-like light cycle of 16 hours of light and eight hours of dark. The second group was born and raised with a cycle of 12 hours of light and 12 hours of dark, like spring and fall. The third group was born and raised in a winter-like light cycle with eight hours of light and 16 hours of dark. Other than the light cycle, the environments were identical.

Depressed mice in water

Scientists have developed a number of tests to determine whether mice are depressed or, more precisely, exhibit depression-like behavior. For example, one of these is a forced swim test, where the researchers put a mouse in a pool of water and measure how much time it spends struggling to get out and how much time it spends floating passively. They argue that a depressed mouse will spend less time struggling than a normal mouse. (Mice float, so they aren’t harmed when they stop paddling.)

Administering several of these tests, the Vanderbilt researchers found that the summer-light-cycle mice exhibited lower levels of depression-like behavior than their spring/fall- or winter-light-cycle counterparts.

When the biologists examined the brains of individuals from the three groups, they found a story consistent with the behavioral testing. They found that serotonergic neurons fire faster in the summer-light-cycle mice and they had elevated levels of serotonin and the neurotransmitter norepinephrine, which is known to excite serotonergic neurons.

“Before, we thought serotonin was probably involved. Now we know that serotonergic neurons are definitely involved,” says Professor Douglas McMahon, who supervised the study.

Intriguingly, when mice born in summer light cycles were switched to winter light cycles the increased firing of serotonin neurons persisted for several months, into adulthood for the mice.

“This showed that early life seasonal photoperiods can have enduring effects on the serotonin neurons. If such an effect occurs in humans, and is long-lasting, it could contribute to the season of birth modulation of SAD risk,” says McMahon.

What about melatonin?

The researchers also had an opportunity to test the role of melatonin. Normally, the hormone affects the serotonergic neurons in the raphe nucleus by binding to a special structure on the neurons’ surface called melatonin receptor 1.

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So the researchers got a strain of genetically engineered mice with melatonin receptor 1 “knocked out.” Although the pineal glands in these mice worked normally, their serotonergic neurons couldn’t respond to the melatonin because they lacked the proper receptors.

When they raised the knock-out mice in the three different photoperiods, the researchers found that the seasonal effect disappeared.

The study appears in the journal Current Biology and was funded by grants from the National Institutes of Health and the Vanderbilt Silvio S. Conte Center.

Source: Vanderbilt University