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The rapid-cooling process uses liquid carbon dioxide to stabilize the proteins in egg whites so much that they could be rated AA—the highest grade for eggs—for 12 weeks. Eggs cooled under current methods lose the AA grade in about half the time.

Earlier research showed that the same cooling technology could significantly reduce occurrences of salmonella illnesses.

“There is no statistical difference in quality between eggs as measured by Haugh units just after laying and rapidly cooled eggs at 12 weeks,” says Kevin Keener, professor of food science at Purdue University.

“This rapid-cooling process can provide a significant extension in the shelf life of eggs compared to traditional processing.” Haugh units measure an egg white’s protein quality.

The results, published in the journal Poultry Science, also show that membranes surrounding the eggs’ yolks were maintained for 12 weeks when eggs were rapidly cooled. That membrane is a barrier that keeps harmful bacteria from reaching the yolk, a nutrient-rich reservoir that bacteria could use as a food source.

“The structural integrity of the yolk membrane stays strong longer, which may provide a food safety benefit,” Keener says. “The membrane being stronger would be another defense against bacterial invasion, such as salmonella.”

The rapid-cooling technology takes liquid carbon dioxide and turns it into a “snow” to rapidly lower the eggs’ temperature. Eggs are placed in a cooling chamber and carbon dioxide gas at about minus 110 degrees Fahrenheit is generated. The cold gas is circulated around the eggs and forms a thin layer of ice inside the eggshell. After treatment, the ice layer melts and quickly lowers an egg’s internal temperature to below 45 degrees, the temperature at which salmonella can no longer grow.

Keener’s previous research showed that the carbon dioxide in bicarbonate form significantly increases the activity of lysozyme, an enzyme in the egg white that has bactericidal properties.

Traditionally, eggs are at more than 100 degrees when placed into a carton. Thirty dozen eggs are then packed in a case, and 30 cases are stacked onto pallets and placed in refrigerated coolers. The eggs in the middle of the pallet can take up to 142 hours—nearly six days—to cool to 45 degrees, Keener says.

A 2005 U.S. government report showed that if eggs were cooled and stored at 45 degrees within 12 hours of laying, there would be about 100,000 fewer salmonella illnesses from eggs in the nation each year. Rapid cooling could also increase the places where eggs could be exported, Keener says.

“You could send eggs anywhere in the world if you could get even eight weeks of shelf life at AA quality. We’re seeing 12 weeks. Right now, you can’t ship eggs anywhere in the world and expect to retain that quality.”

With additional funding he would like to continue to study the benefits of rapid cooling, including inoculating the inside of shell eggs with Salmonella and examining how other proteins in the whites and yolks of eggs are affected.

Keener is a technical consultant to the American Egg Board and a member of the United Egg Producers Scientific Advisory Panel. His work was funded by Purdue and gift funds.

More news from Purdue University: http://www.purdue.edu/newsroom/