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How piglets could make IVF cheaper

Scientists have made a discovery in piglets that could decrease the costs associated with in vitro fertilization in people.

Parents seeking to have children through IVF spend between $12,000 and $15,000 each session plus the cost of medications, which could average between $3,000 and $5,000.

Scientists were working with pigs to research stem cells and the mechanisms they use to proliferate, communicate, and grow in the body. During an attempt to improve how they grow the cells, researchers discovered a method that uses a special liquid medium that improves the success of IVF in pigs.

“It was a serendipitous discovery, really,” says R. Michael Roberts, professor of animal sciences at the University of Missouri. “Generally, there are multiple steps to producing viable embryos that we can then implant in pigs and cows involved in our research; however, it’s costly and sometimes yields very little return.

“We were seeking a way to do that more efficiently and stumbled upon a method that may have implications in human fertility clinics as well.”

In IVF involving pigs, scientists first extract oocytes, or eggs from female pigs as well as the “nurse” cells that surround them and place them in a chemical environment designed to mature the eggs. The eggs are then fertilized to create zygotes, or single-celled embryos that are allowed to develop for six days. These embryos are then transferred back into a female pig with the hope of achieving a successful pregnancy and healthy piglets.

“The chance of generating a successful piglet after all those steps is very low; only 1-2 percent of the original oocytes make it that far,” Roberts says. “Normally, researchers overcome this low success rate by implanting large numbers of embryos, but that takes a lot of time and money.”

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Ye Yuan, a former research assistant professor in Roberts’ lab, and Lee Spate, a senior research specialist in animal sciences, set out to increase the efficiency and quality of piglet embryos before they are implanted.

In one study, the team analyzed various special growth factors used when culturing pig stem cells and added two factors—fibroblast growth factor 2 (FGF2) and leukemia inhibitory factor (LIF). When added with a third factor—insulin-like growth factor—the combination created the special fluid environment the oocytes needed to become competent for fertilization and further development to embryos that could provide a successful pregnancy.

Together, the three compounds create the chemical medium called “FLI,” which could revolutionize both piglet and human IVF treatments.

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“It improved every aspect of the whole process and almost doubled the efficiency of oocyte maturation,” Roberts says. “Whenever you’re doing science, you’d like to think you’re doing something that could be useful.

“When we started it wasn’t to improve fertility IVF in women, it was to just get better oocytes in pigs. Now it’s possible that FLI medium could become important in bovine embryo work and possibly even help with human IVF.”

The findings appear in the Proceedings of the National Academy of Sciences. A patent application has been filed through the university’s Office of Technology Management and Industry Relations to encourage commercialization of the new method.

The National Institutes of Health and the University of Missouri Food for the 21st Century Program supported the work. The content is solely the responsibility of the authors and does not necessarily represent the official views of the funding agencies.

Source: University of Missouri

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