While trying to grow placenta cells, researchers made a surprising discovery: a previously unknown form of human embryonic stem cell.
“These new cells, which we are calling bone morphogenetic protein (BMP)-primed stem cells, are much more robust and easily manipulated than standard embryonic stem cells,” says R. Michael Roberts, a professor at the University of Missouri.
He says the cells represent a transition between embryonic stem cells and their ultimate fate, for example as placenta, brain, or skin cells.
“We can use these new stem cells for future research to better understand how embryos are organized and what causes diseases like pre-eclampsia and other prenatal problems,” adds Roberts.
Embryonic stem cells are pluripotent, meaning they can develop into a number of different types of cells. For their study, Roberts and colleagues were attempting to grow placenta cells from embryonic stem cells by adding a substance called BMP-4 for a shorter period of time than had been done previously. They also added two other drugs that temporarily inhibited key biochemical pathways associated with the pluripotent state of the stem cells.
Instead of forming placenta cells, the stem cells grew into what was a previously unobserved state, referred to by the scientists as “BMP primed” stem cells.
They found these cells to be much easier to work with in a laboratory setting than traditional stem cells because they are easier to grow and are more uniform, meaning that all the cells in the culture are quite similar to each other in the way they express their genetic information.
“Previously, the common thought was that embryonic stem cells transitioned straight from stem cells to their end products,” Roberts says. “These new stem cells made us realize that embryonic stem cells exist in a number of different transitional states, which likely resemble those encountered in the early stages of embryos.
“This should open the door for future stem cell research that is much more efficient. We now have new stem cells that are easier to manipulate since they are already at the key transitional precipice before changing into placenta cells, skin cells or any other kind of cell that makes up the human body.”
The study is published in the Proceedings of the National Academy of Sciences.
Source: University of Missouri