Cutting key protein may keep tumors local
PENN STATE (US) — Targeting a protein that transports cargo within cells may slow the spread of cancer, according to researchers.
The motor protein km23-1 is also involved in the migration of cells. Migration is necessary for cancer to spread, so understanding cell movement is important for the development of better cancer treatments.
Kathleen Mulder, professor of biochemistry and molecular biology at Penn State, looked for partner proteins that bind to and cooperate with km23-1 during cell movement, which turned out to include factors that can control proteins actin and RhoA.
“Cell migration is an important aspect of the process of a tumor spreading,” says Mulder. “Changes in this process transform tumor cells from local, noninvasive, confined cells to the migrating, metastatic cancer cells.”
Cells move through the body using the protein actin, which forms the structural frame of the cell, called the cytoskeleton. The actin creates a protrusion in the cell membrane by forming strands of thread-like fibers on the leading edge of the cell, pushing the cell forward. Several identified proteins regulate the reorganization of the cytoskeleton and are more active in several types of cancers.
Overexpression of km23-1 increases actin fiber formation, whereas when km23-1 is diminished, RhoA activity decreases. RhoA is known to be an important switch, activating processes in migration.
“By knowing that RhoA activity was decreased when km23-1 was reduced, we infer that km23-1 is needed for the regulation of these switches and has a role in cell movement,” explains Mulder. She and colleagues report the findings in Biochemical and Biophysical Research Communications.
To test this in the lab, km23-1 was reduced in a sample of human colon cancer cells. When km23-1 was diminished, cancer cells migrated less. More research needs to be done, but km23-1 may be a promising target for anti-metastatic drugs and cancer therapies to slow the spread of the disease.
“By inhibiting km23-1, you inhibit events that contribute to the cells spreading to other parts of the body,” says Mulder.
The National Institutes of Health funded the study.
Source: Penn State
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