For the first time, researchers may be able to use mice as a test bed for treatments aimed at shrinking children’s tumors.
Unlike adults with brain cancer, children cannot receive radiation therapy, so doctors need to rely on medications and other strategies. Brain tumors are the leading cause of cancer death in children. A lack of treatment options keeps survival rates low.
“This is exciting because it’s the first animal model of pediatric high-grade gliomas, or malignant brain tumors,” says Maria Castro, professor in the neurosurgery and cell and developmental biology departments at the University of Michigan. “The mice carry the genetic mutations found in human tumors, and develop tumors that closely resemble what children and adolescents do.”
And unlike previous attempts by others, the new model has a fully functional immune system, which makes it even more like the children they mimic.
In addition to developing the new genetically engineered mouse model for the form of cancer called glioblastoma multiforme, researchers made a key discovery about brain tumor biology via the mice. Their work, published in the journal Science Translational Medicine, focuses on a protein called ATRX, and its role in helping cells repair damage to DNA. About one-third of children and young adults with brain cancer have ATRX mutations in their cancer cells.
But in an ironic—and potentially beneficial—twist, the findings also showed that if they treated the mice’s tumors with drugs that damage DNA, they could actually shrink tumors more effectively and improve survival. The genetic instability accelerated tumor growth and reduced the survival of mice that went without treatment.
Using a special genetic technique that introduced the same mutation in mice, soon after birth, the scientists were able to generate brain tumors that made less of the ATRX protein. Then, they showed that when the cancer cells didn’t make enough ATRX, the cells couldn’t join together the two ends of a broken DNA strand.
DNA-damaging cancer drugs already exist, and are used for other purposes in adults. If further research in the mice bears out, they could soon be tested in children whose brain tumors also show a decrease in ATRX production.
“We are very excited about this tumor model as it mimics the developmental environment of a pediatric or adolescent human brain tumor,” says children’s cancer specialist Carl Koschmann. “We desperately need new therapies for pediatric GBM patients, as less than 20 percent of children diagnosed with GBM will survive five years. We currently base their treatment on a regimen designed for adult patients with GBM, which are very different tumors at the molecular level. Our mouse model has given us a great step towards developing targeted therapies to specific changes found in pediatric and adolescent GBM.”
A crucial aspect of the mouse model development was the ability to use what’s called the “Sleeping Beauty” technique of inserting genes into stem cells in the brain of newborn mice. This allows their brain to develop normally, but also triggers the development of cancer by adding mutated ATRX genes and other genes known to be involved in cancer.
They also looked at genetic data on several hundreds of adult and pediatric brain tumors from around the world. That helped them confirm that mutations in the gene that directs cells to make ATRX are responsible for making the tumors genetically unstable.
Personalizing treatment for brain tumors based on an individual’s ATRX mutation status is still a concept, not a reality. But the researchers believe their mouse model and the close collaboration with pediatric brain cancer treatment teams will accelerate the ability to do so.
Other research at U-M is developing new options for treating brain cancer through immunotherapy—harnessing the immune system to attack cancer cells once an injection of a particular gene therapy is delivered into the brain tumor. A clinical trial testing an immunotherapy approach developed in the lab of study coauthor Pedro Lowenstein and Maria Castro is now under way in adults with GBM.
Researchers are also working to discover new antigens that the ATRX-deficient brain tumor cells may have. Once they find such tumor neo-antigens, as they’re called, they could design other immunotherapy strategies that could target them. They also hope to work with pharmaceutical companies to test existing or new drugs in the mice, and determine if they could work against ATRX-mutated brain tumors.
“The technique could be tested in children, too, if research involving the new mouse model shows it might be successful. That’s why having a fully functioning immune system in the mice is especially crucial.”
Researchers from Johns Hopkins School of Medicine, the University of Rochester, and the Institute of Cancer Research in London are coauthors of the study. The National Institute for Neurological Diseases and Stroke, the National Cancer Institute, the St. Baldrick’s Foundation Fellowship, and an Alex’s Lemonade Stand Foundation/Northwestern Mutual Young Investigator Grant funded the work.
Source: University of Michigan