Bacteria build ‘houses’ in healthy cells
PURDUE (US) — Bacteria are able to build camouflaged homes for themselves inside healthy cells—and cause disease—by manipulating a natural cellular process, new research shows.
Published in Proceedings of the National Academy of Sciences, the study reveals how a pair of proteins from the bacteria Legionella pneumophila, which causes Legionnaires’ disease, alters a host protein in order to divert raw materials within the cell for use in building and disguising a large structure that houses the bacteria as it replicates.
The modification of the host protein creates a dam, blocking proteins that would be used as bricks in cellular construction from reaching their destination, says Zhao-Qing Luo, associate professor of biological sciences at Purdue University.
The protein “bricks” are then diverted and incorporated into a bacterial structure called a vacuole that houses bacteria as it replicates within the cell. Because the vacuole contains materials natural to the cell, it goes unrecognized as a foreign structure.
“The bacterial proteins use the cellular membrane proteins to build their house, which is sort of like a balloon,” Luo says.
“It needs to stretch and grow bigger as more bacterial replication occurs. The membrane material helps the vacuole be more rubbery and stretchy, and it also camouflages the structure. The bacteria is stealing material from the cell to build their own house and then disguising it so it blends in with the neighborhood.”
The method by which the bacteria achieve this theft is what was most surprising to Luo.
The bacterial proteins, AnkX and Lem3, modify the host protein through a biochemical process called phosphorylcholination that is used by healthy cells to regulate immune response.
The process is known to happen in many organisms and involves adding a small chemical group, called the phosphorylcholine moiety, to a target molecule, he says.
Luo and colleagues discovered that AnkX adds the phosphorylcholine moiety to a host protein involved in moving proteins from the cell’s endoplasmic reticulum to their cellular destinations. The modification effectively shuts down this process and creates a dam that blocks the proteins from reaching their destination.
The bacterial protein Lem3 is positioned outside the vacuole and reverses the modification of the host protein to ensure that the protein “bricks” are free to be used in creation of the bacterial structure.
The study was the first to identify proteins that directly add and remove the phosphorylcholine moiety, Luo says.
“We were surprised to find that the bacterial proteins use the phosphorylcholination process and to discover that this process is reversible,” he says. “This is evidence of a new way signals are relayed within cells, and we are eager to investigate it.”
The team also found that the phosphorylcholination reaction is carried out at a specific site on the protein called the Fic domain. Previous studies had shown this site induced a different reaction called AMPylation.
It’s rare for a domain to catalyze more than one reaction, and it was previously thought that the site’s only responsibility was to transfer the chemical group necessary for AMPylation.
“Revealing that this domain has dual roles is very important to identify or screen for compounds to inhibit its activity and fight disease,” Luo says. “This domain has a much broader involvement in biochemical reactions than we thought and may be a promising target for effective treatments.”
During infection, bacteria deliver hundreds of proteins into healthy cells that alter cellular processes to turn the hostile environment into one hospitable to bacterial replication, but the specific roles of only about 20 proteins are known, Luo says.
“In order to pinpoint proteins that would be good targets for new antibiotics, we need to determine their roles and importance to the success of infection,” he says.
“We need to understand at the biochemical level exactly what these proteins do and how they take over natural cellular processes. Then we can work on finding ways to block these activities, stop the infection, and save lives.”
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