PNAS Cover image.tif

After 3 days, lung bacteria are unstoppable

UNC-CHAPEL HILL (US) — A deadly plague bacterium is able to transform the lungs into a breeding ground for other microbes—often escaping detection until it is too late for medical treatment.

Most other microbes that infect the lungs trigger an antimicrobial response within a few hours after infection. This early inflammatory response is generally sufficient to eliminate microorganisms with no more than mild respiratory symptoms. But the pneumonic plague bacterium, Yersinia pestis, hides out for about 36 hours when the lungs are “quiet,” not inflamed, and symptoms are completely absent.

During that first 36 hours of infection, plague bacteria are having a field day, growing and reproducing rapidly—2-fold, 100-fold, 100,000-fold—and all without outward disease symptoms or measurable changes in lung tissue.

“And then, rather abruptly, symptoms start to appear,” says William Goldman, professor of microbiology and immunology at the University of North Carolina at Chapel Hill. “They progress rapidly to the point where you realize this is not just a cold, this is not just the flu. But by then the disease has progressed too far for effective medical intervention, and death is likely within the next day or two.”

And once people have pneumonic plague, the bacteria can spread via respiratory droplets to others who have close contact with them. The U.S. Centers for Disease Control and Prevention notes that during the delay between being exposed to Y. pestis and becoming seriously sick, people could travel over a large area, possibly infecting others, which could make the infection more difficult to control.

“Here’s the question we wanted to answer: Is the organism avoiding detection or is it actually suppressing the immune responses of the lung?” Goldman says. “The paper is really about the experiments designed to distinguish between these possibilities. And the answer we found suggests the latter.”

As reported in Proceedings of the National Academy of Sciences, in their “co-infection” experiments, the study team mixed together a fully virulent Y. pestis strain and a mutant strain known not to be infectious in that it lacked the components essential for it to be a pathogen. The mix was then given to a single laboratory animal.

“The expectation would be that the virulent strain would do an excellent job of infecting the host. And the non-virulent strain would get killed by the host,” Goldman says. “But in our experiments, the non-virulent strain would actually grow very well, almost as well as the virulent strain, and we would see this with any non-virulent strain of Y. pestis.”

The study team then tried other microbes, different lung pathogens, and an assortment of random microbes—“including the sort of organisms you inhale all the time and that are disposed of easily by the lungs’ standard defense mechanisms. But as long as the virulent bacteria were present, the non-virulent organisms would grow,” he says.

“There is no other microbe that does that, no other inhaled organism that in a matter of minutes or hours transforms the lung into such a permissive environment for microbial proliferation.”

Not much evolutionary distance exists between Yersinia pestis and its closest ancestor, Yersinia pseudotuberculosis, which causes a much milder disease, Goldman says.

“Our work shows that of these two species, only Y. pestis has the ability to transform the lung into an environment that permits an extended period of unrestricted microbial proliferation with no symptoms.  Looking at the genetic differences between these two species may reveal the mechanism responsible for this phenomenon exclusive to Y. pestis, and that may lead to new therapeutic strategies for pneumonic plague.”

Support for the research came from a National Institutes of Health grant to the Southeastern Regional Center of Excellence for Emerging Infections and Biodefense.

More news from UNC-Chapel Hill: http://uncnews.unc.edu/

chat1 Comment

You are free to share this article under the Creative Commons Attribution-NoDerivs 3.0 Unported license.

  1. cortese

    After reading this article I’m terrified from a common flu.

    By the time you realize is not just a cold the lung bacteria has progressed so far that death is likely to occur in couple of days.

    Gosh! Let’s hope to find a way to detect it earlier!

We respect your privacy.